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Salmonella enterotoxin (Stn) regulates membrane composition and integrity


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タイトル: Salmonella enterotoxin (Stn) regulates membrane composition and integrity
著者: Nakano, Masayuki / Yamasaki, Eiki / Ichinose, Akitoyo / Shimohata, Takaaki / Takahashi, Akira / Akada, Junko K. / Nakamura, Kazuyuki / Moss, Joel / Hirayama, Toshiya / Kurazono, Hisao
発行日: 2012年 7月
出版者: Company of Biologists Ltd
引用: Disease Models & Mechanisms, 5(4), pp.515-521; 2012
抄録: The mechanism of action of Salmonella enterotoxin (Stn) as a virulence factor in disease is controversial. Studies of Stn have indicated both positive and negative effects on Salmonella virulence. In this study, we attempted to evaluate Stn function and its effects on Salmonella virulence. To investigate Stn function, we first performed in vitro and in vivo analysis using mammalian cells and a murine ileal loop model. In these systems, we did not observe differences in virulence phenotypes between wild-type Salmonella and an stn gene-deleted mutant. We next characterized the phenotypes and molecular properties of the mutant strain under various in vitro conditions. The proteomic profiles of the total cell membrane protein fraction differed between wild type and mutant in that there was an absence of a protein in the mutant strain, which was identified as OmpA. By far-western blotting, OmpA was found to interact directly with Stn. To verify this result, the morphology of Salmonella was examined by transmission electron microscopy, with OmpA localization being analyzed by immunogold labeling. Compared with wild-type Salmonella, the mutant strain had a different pole structure and a thin periplasmic space; OmpA was not seen in the mutant. These results indicate that Stn, via regulation of OmpA membrane localization, functions in the maintenance of membrane composition and integrity.
キーワード: cell membrane protein / CXCL1 chemokine / CXCL2 chemokine / CXCL3 chemokine / enterotoxin / granulocyte macrophage colony stimulating factor / monocyte chemotactic protein 3 / outer membrane protein A / RANTES / salmonella enterotoxin / unclassified drug / amino acid sequence / animal experiment / article / bacterial membrane / bacterial strain / bacterial virulence / controlled study / female / gene deletion / human / human cell / immunogold labeling / in vitro study / in vivo study / mouse / nonhuman / phenotype / priority journal / protein interaction / protein localization / protein structure / proteomics / Salmonella enterica / transmission electron microscopy / Western blotting / wild type
URI: http://hdl.handle.net/10069/29764
ISSN: 17548403
DOI: 10.1242/dmm.009324
権利: © 2012. Published by The Company of Biologists Ltd This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial Share Alike License (http://creativecommons.org/licenses/by-nc-sa/3.0), which permits unrestricted non-commercial use, distribution and reproduction in any medium provided that the original work is properly cited and all further distributions of the work or adaptation are subject to the same Creative Commons License terms
資料タイプ: Journal Article
原稿種類: publisher
出現コレクション:120 学術雑誌論文

引用URI : http://hdl.handle.net/10069/29764

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