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コイ腹腔滲出細胞からのマクロファージの調製


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Title: コイ腹腔滲出細胞からのマクロファージの調製
Other Titles: Preparation of Macrophage Monolayer from Carp Peritoneal Exudate Cells
Authors: 原, 研治 / 槌本, 六秀 / 橘, 勝康 / 長富, 潔 / 古場, 久代 / 石原, 忠
Authors (alternative): Hara, Kenji / Tsuchimoto, Mutsuhide / Tachibana, Katsuyasu / Osatomi, Kiyoshi / Koba, Hisayo / Ishihara, Tadashi
Issue Date: Dec-1993
Publisher: 長崎大学水産学部
Citation: 崎大学水産学部研究報告, v.74・75, pp.51-57; 1993
Abstract: We investigated the preparation of macrophage monolayer from carp peritoneal exudate cells (PEC) to clarify the biosynthesis and processing of cathepsins. PEC collected at 24h interval after elicitation with 6% sodium caseinate or thioglycolate medium were transferred to culture dish in RPMI 1640 (RPMI), and the cells were incubated in CO₂ incubator for 2h. To distinguish macrophage and neutrophil, the adherent cells washed with RPMI to remove non-adherent cells were analyzed histochemically by the staining of two different esterase activities. The number of PEC was maximally increased at 3 days after injection with 15 ml of sodium caseinate, whereas that of macrophages was at 4 days after injection. Most. (about 90%) of cells cultured for 24h were positive for α-Naphthyl butyrate esterase activity but they were not positive for Naphthol AS-D chloroacetate esterase activity, confirming them to be macrophage. They were able to culture for 48h in RPMI containing 5% heat inactivated calf serum. It was possible to prepare two culture dishes of macrophage monolayer (1.33 x 10⁵/cm²) from one carp (800-900g) by this method.
Keywords: マクロファージ / macrophage / 腹腔滲出細胞 / peritoneal exudate cells / 好中球 / neutrophil / コイ / carp / 非特異性エステラーゼ / non-specific esterase
URI: http://hdl.handle.net/10069/29831
ISSN: 05471427
Type: Departmental Bulletin Paper
Text Version: publisher
Appears in Collections:第74,75号

Citable URI : http://hdl.handle.net/10069/29831

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