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Determination of glyoxylic acid in urine by liquid chromatography with fluorescence detection, using a novel derivatization procedure based on the Petasis reaction


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Title: Determination of glyoxylic acid in urine by liquid chromatography with fluorescence detection, using a novel derivatization procedure based on the Petasis reaction
Authors: Chihara, Kohki / Kishikawa, Naoya / Ohyama, Kaname / Nakashima, Kenichiro / Kuroda, Naotaka
Issue Date: Jul-2012
Publisher: Springer Verlag
Citation: Analytical and Bioanalytical Chemistry, 403(9), pp.2765-2770; 2012
Abstract: The Petasis reaction is the multi-component reaction of a carbonyl compound, amine, and arylboronic acid to form an α-amino acid or a β-aminoalcohol. In this work, as the first analytical application of the Petasis reaction, a highperformance liquid chromatographic (HPLC) method with fluorescence detection was developed for determination of glyoxylic acid. The glyoxylic acid was derivatized with 1- pyreneboronic acid, as fluorescent arylboronic acid, in the presence of N-methylbutylamine, as amine, to give a fluorescent α-amino acid. HPLC separation of the fluorescent derivative was performed within 30 min on an octyl column eluted with a gradient prepared from acetonitrile and 50 mmol L -1 acetate buffer (pH 4.0). The detection limit (S/N03) for glyoxylic acid was 5.0 nmol L -1 (20 fmol/injection). The method can be used to determine the concentration of glyoxylic acid in human urine without interference from biological components.
Keywords: Acetate buffers / Amino alcohols / Analytical applications / Arylboronic acids / Biological components / Derivatization procedures / Detection limits / Fluorescence derivatization / Fluorescence detection / Glyoxylic acids / HPLC separation / Human urine / Multi-component reactions / Petasis reaction
URI: http://hdl.handle.net/10069/29900
ISSN: 16182642
DOI: 10.1007/s00216-012-6036-1
Rights: © Springer-Verlag 2012. / The final publication is available at www.springerlink.com.
Type: Journal Article
Text Version: author
Appears in Collections:Articles in academic journal

Citable URI : http://hdl.handle.net/10069/29900

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