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蛍光・化学発光反応に基づく高選択的検出法の開発と生体分析への応用


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Title: 蛍光・化学発光反応に基づく高選択的検出法の開発と生体分析への応用
Other Titles: Development of Selective Detection Methods for Pharmaceutical and Biological Compounds Based on Fluorescence and Chemiluminescence Techniques and Its Application for Biomedical Analyses
Authors: 岸川, 直哉
Authors (alternative): Kishikawa, Naoya
Issue Date: 27-Jul-2012
Publisher: 日本分析化学会 / Japan Society for Analytical Chemistry
Citation: 分析科学, 61(7), pp.583-590; 2012
Abstract: 生体内に存在する医薬品やバイオマーカーの濃度を測定することは,治療効果の確認や疾患の診断のために重要である.しかしながら,生体に由来する試料中には夾 きょう 雑物となる生体成分が数多く存在し,これらが測定対象の検出を妨害するという問題点が存在している.著者らは,複雑な生体成分が共存する生体内から,微量の医薬品やバイオマーカーのみを特異的に検出するために,測定対象化合物に特徴的な構造あるいは性質を利用する高選択的かつ高感度な蛍光・化学発光分析法の開発を行い,生体分析の分野においてその有用性を明らかにしてきた.本論文ではその中でも中心的な二つの手法,(1)パラジウムカップリング反応に基づくアリールハライド及び末端二重結合の選択的蛍光誘導体化法及び(2)キノンの選択的蛍光・化学発光定量法について紹介する. / In order to provide therapeutic advice or diagnosis, it is important to determine the concentration of a pharmaceutical agent or biomarker in biological fluids. However, it is difficult to determine a target analyte selectively due to interferences derived from a large number of co-existing biological components. In order to overcome this problem, we have developed selective detection methods for pharmaceutical agent or biomarker in the presence of biological components using a specific structure or characteristic of the target analyte. Among related studies, this review describes two principle methods : namely (1) selective fluorescence derivatization methods for aryl halide or terminal double bond based on palladium coupling reaction, and (2) selective detection methods for quinone by chemiluminescence and fluorescence reactions of utilizing the specific characteristics of quinone.
Keywords: fluorescence / chemiluminescence / high-performance liquid chromatography / palladium coupling reaction / quinone
URI: http://hdl.handle.net/10069/30256
ISSN: 05251931
DOI: 10.2116/bunsekikagaku.61.583
Rights: © The Japan Society for Analytical Chemistry 2012
Type: Journal Article
Text Version: publisher
Appears in Collections:Articles in academic journal

Citable URI : http://hdl.handle.net/10069/30256

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