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数種海藻からのプロトプラストの分離と培養


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Title: 数種海藻からのプロトプラストの分離と培養
Other Titles: Isolation and Culture of Protoplasts from Some Seaweeds
Authors: 藤田, 雄二 / 右田, 清治
Authors (alternative): Fujita, Yuji / Migita, Seiji
Issue Date: Feb-1985
Publisher: 長崎大学水産学部
Citation: 長崎大学水産学部研究報告, v.57, pp.39-45; 1985
Abstract: Protoplasts were isolated, from vegetative thalli of Ulva pertusa (general strain and sterile mutant strain), Enteromorpha linza and Monostroma nitidum (Chlorophyta) following digestion with Cellulase Onozuka R-10. Protoplasts from vegetative thalli of Uudaria pinnatifida (Phaeophyta) and Porphyra yezoensis (Rhodophyta) were obtained by using a crude enzyme mixture prepared from the cultured supernatant fraction of Pseudomonas strain P-1 which was isolated from cultivated Porphyra thallus. After 10 days of culture in Provasoli's enriched sea water medium, about 41-90% of the initial number of protoplasts from three species of Chlorophyta and P. yezoensis regenerated cell walls. The cells which regenerated cell walls in general strain of U. pertusa, E. linza and M. nitidum generally formed and discharged gametes or zoospores, and their germlings except M. nitidum normally developed into thalli. Most of the cells in the sterile mutant strain of U. pertusa and some of cells in E. linza, which regenerated cell walls, underwent cell division and grew into spherical or subsherical thalli which were composed of a single layer of cells. After regeneration of cell walls, the cells of P. yezoensis underwent cell division, formed rhizoids, and grew into thalli of circular or irregular shapes. The thalli formed and liberated monospores and their germlings grew into thalli of normal shapes. However, protoplasts of U. pinnatifida did not regenerate cell walls after 10 days culture. These results show the possibility of using developable protoplasts in the investigation of physiological and genetic problems of seaweeds.
URI: http://hdl.handle.net/10069/30388
ISSN: 05471427
Type: Departmental Bulletin Paper
Text Version: publisher
Appears in Collections:第57号

Citable URI : http://hdl.handle.net/10069/30388

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