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NAOSITE : Nagasaki University's Academic Output SITE > Faculty of Fisheries > Bulletin > Bulletin of the Faculty of Fisheries > 第48号 >

培地還元剤としてのチオグリコール酸ナトリウムおよびL-アスコルビン酸ナトリウムがウェルシュ菌NCTC 8238の胞子形成およびエンテロトキシン産生におよぼす影響


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Title: 培地還元剤としてのチオグリコール酸ナトリウムおよびL-アスコルビン酸ナトリウムがウェルシュ菌NCTC 8238の胞子形成およびエンテロトキシン産生におよぼす影響
Other Titles: Influence of Sodium Thioglycollate and Sodium L-Ascorbate as Medium Reducing Agents on Sporulation and Enterotoxin Production of Clostridium perfringens NCTC 8238
Authors: 谷口, 忠敬 / 橘, 勝康
Authors (alternative): Taniguti, Tadataka / Tachibana, Katsuyasu
Issue Date: Feb-1980
Publisher: 長崎大学水産学部
Citation: 長崎大学水産学部研究報告, v.48, pp.35-39; 1980
Abstract: The influences of addition of sodium thioglycollate (TGC) and sodium L-ascorbate (ASC) as medium reducing agents on the sporulation and enterotoxin (ET) production of Clostridium perfringens NCTC 8238 have been studied. 1) In the case of TGC addition, ET production was positive in the cultures with 0.1 and 0.5% TGC, but it was negative in the cultures with 1.0 and 1.5% TGC. The rate of sporulation and the number of spores decreased distinctly by the addition of 1.0 and 1.5% TGC. The number of heat-resistant (100℃, 10 min) spores was high in the culture with 0.1 TGC, and it decreased with increasing the concentration of TGC above 0.5%. 2) In the case of ASC addition, ET production was recognized in the cultures with ASC in the range from 0.1 to 1.5%, and it was maximum in the culture with 0.5% ASC. There was no significant difference among the numbers of heat-resistant spores in the cultures with ASC from 0.1 to 1.5%. 3) The amount of ET produced, sporulation rate, number of spores and number of heatresistant spores in the culture with 0.5% ASC were the same as or higher than those with 0.1% TGC. / 培地還元剤としてのチオグリコール酸ナトリウム(TGC)およびL-アスコルビン酸ナトリウム(ASC)の添加がウェルシュ菌NCTC 8238のエンテロトキシン(ET)産生および胞子形成におよぼす影響を比較した. 1)TGC添加の場合,ET産生はTGC0.1および0.5%添加で認められ,1.0および1.5%添加では陰性であった.胞子形成率および胞子数も1.0および1.5%添加で著しく低下した.耐熱性胞子数は0.1%添加で高く,添加濃度の増加(0.5%以上)とともに低下した. 2)ASC添加の場合,ET産生はASC0.1~1.5%添加範囲で認められ,0.5%添加で最高値を示した.胞子形成率および胞子数も0.5%添加で最高値を示した.耐熱性胞子数は0.1~1.5%添加範囲では大差は認められなかった. 3)ASC 0.5%添加におけるET産生量,胞子形成率,胞子数および耐熱性胞子数はTGC 0.1%添加におけるそれぞれ以上であった.
URI: http://hdl.handle.net/10069/30574
ISSN: 05471427
Type: Departmental Bulletin Paper
Text Version: publisher
Appears in Collections:第48号

Citable URI : http://hdl.handle.net/10069/30574

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