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Title: 蛍光法によるクロロフィルa測定に必要な定数算出のための色素の単離法
Other Titles: On the Method of Isolation of Chlorophyll-a and Phaeophytin-a for Determination of two Constant Numerals in Fluorometric Chlorophyll-a Measurement
Authors: 伊藤, 栄樹 / 飯塚, 昭二
Authors (alternative): Itoh, Hideki / Iizuka, Shoji
Issue Date: Aug-1979
Publisher: 長崎大学水産学部
Citation: 長崎大学水産学部研究報告, v.47, pp.1-4; 1979
Abstract: The fluorometric method of measuring chlorophyll-a involves some difficulties at practical measurement by field scienists unfamiliar with chemical analysis, although this method has some advantages. One of the problems is the difficulty in isolating pure chlorophyll-a and/or phaeophytina. This paper describes a simplified method of isolation of chlorophyll-a and phaeophytin-a for determination of two constants (fph and acid factor) without any special equipment or aid of skilled technique. Three features of this simplified method are mentioned in this paper. The first is to use fresh "wakame" (Undaria pinnatifida, brown alga) containing chlorophyll-a and no chlorophyll-b which is otherwise difficult to separate from chlorophyll-a; the second is to determine the amount of phaeophytin-a by light absorption method in order to simplify chromatography treatment; and the third is to isolate chlorophyll-a by paper chromatography.
URI: http://hdl.handle.net/10069/30610
ISSN: 05471427
Type: Departmental Bulletin Paper
Text Version: publisher
Appears in Collections:第47号

Citable URI : http://hdl.handle.net/10069/30610

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