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シオミズツボワムシのプロテアーゼに関する研究-1 : 粗酵素液中のプロティナーゼ活性の性質について


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Title: シオミズツボワムシのプロテアーゼに関する研究-1 : 粗酵素液中のプロティナーゼ活性の性質について
Other Titles: Studies on Protease of the Rotifer, Brachionus plicatilis―I Some Properties of Proteinase in the Crude Extracts
Authors: 原, 研治 / 石原, 忠 / 荒野, 拓 / 保田, 正人
Authors (alternative): Hara, Kenji / Ishihara, Tadashi / Arano, Hiraki / Yasuda, Masato
Issue Date: Feb-1979
Publisher: 長崎大学水産学部
Citation: 長崎大学水産学部研究報告, v.46, pp.31-35; 1979
Abstract: Acid and alkaline proteinase activities were detected in an ammonium sulfate fraction (50-80%) of crude extracts from the rotifer, Brachionus plicatilis. Some properties of the activities in the fraction were investigated. The optimum pH was obtained at about 4.0 for acid proteinase and at about 8.0 for alkaline one. The optimum temperatures of the acid and the alkaline proteinase were 60 and 50℃, respectively, under the experimental condition. The both proteinase activities were unstable as heated at 60℃ for 10 minutes. The acid proteinase activity was inhibited with 1×10⁻³M of Hg²⁺, Cu²⁺, Ag⁺, I₂, monoiodoacetate and PCMB. The alkaline proteinase activity was inhibited with 1×10⁻³M of Hg²⁺, Cu²⁺, Ag⁺, I₂, NBS, DFP, TLCK and potassium permangate.
URI: http://hdl.handle.net/10069/30649
ISSN: 05471427
Type: Departmental Bulletin Paper
Text Version: publisher
Appears in Collections:第46号

Citable URI : http://hdl.handle.net/10069/30649

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