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A Simple and Rapid Determination of Alginate Lyase Activity


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タイトル: A Simple and Rapid Determination of Alginate Lyase Activity
その他のタイトル: アルギン酸リアーゼの活性の簡易測定法
著者: Muramatsu, Tsuyoshi
著者(別表記) : 村松, 毅
発行日: 1975年12月
出版者: 長崎大学水産学部
引用: 長崎大学水産学部研究報告, v.40, pp.35-38; 1975
抄録: A simple and rapid method for determination of alginate lyase activity has been investigated only by following the increase in absorbance in ultraviolet region of the reaction mixture. Partially purified enzyme from wreath shell (Turbo cornutus) acted on alginic acid, giving good linearity of the absorbance at 235 nm against the reaction time up to about 5min just after the initiation of the enzyme reaction under the condition applied. The initial reaction velocity can be easily obtained from the straight line in a simple operation. Alginate lyase 〔EC 4.2.99.4〕 acts on alginic acid, which is suggested to consist of mannuronic and guluronic acid, liberating uronides and uronic acid monomers with reducing end group and double bond on C4-C5 linkage of a pyranose ring at the site of the action. Based on the reducing power and the spectrophotometric or hydrodynamic properties of the reaction products, various methods for determination of alginate lyase activity have been reported. These methods are laborious, time-consuming and less useful for general kinetic analysis of enzyme action. In the experiments described in this paper, a simple and rapid method has been investigated only by following the increase in absorbance in ultraviolet region originated in formation of the double bond (eliminase action). Similar methods have been reported by Nakada et al., Preiss and Ashwell and Nakada and Sweeny for investigation of eliminase.
URI: http://hdl.handle.net/10069/30761
ISSN: 05471427
資料タイプ: Departmental Bulletin Paper
原稿種類: publisher
出現コレクション:第40号

引用URI : http://hdl.handle.net/10069/30761

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