DSpace university logo mark
Advanced Search
Japanese | English 

NAOSITE : Nagasaki University's Academic Output SITE > Faculty of Fisheries > Articles in academic journal >

Stimulatory effect of the sulfated polysaccharide ascophyllan on the respiratory burst in RAW264.7 macrophages

File Description SizeFormat
IJBM52_164.pdf476.35 kBAdobe PDFView/Open

Title: Stimulatory effect of the sulfated polysaccharide ascophyllan on the respiratory burst in RAW264.7 macrophages
Authors: Wang, Yajun / Jiang, Zedong / Kim, Daekyung / Ueno, Mikinori / Okimura, Takasi / Yamaguchi, Kenichi / Oda, Tatsuya
Issue Date: Jan-2013
Publisher: Elsevier
Citation: International Journal of Biological Macromolecules, 52, pp.164-169; 2013
Abstract: In this study, we investigated the bioactivity of ascophyllan in terms of reactive oxygen species (ROS) generation. In RAW264.7 cells, we found that ascophyllan induced ROS generation in a concentration-dependent manner, but with bell-shaped profile. Immunoblot analysis demonstrated that ascophyllan promoted the translocation of cytosolic subunits (p67phox and p47phox) of NADPH oxidase to the plasma membrane. Among mitogen activated protein (MAP) kinase inhibitors tested, JNK inhibitor showed the most potent inhibitory effect on ascophyllan-induced ROS production. Consistently, significant level of phosphorylated JNK MAP kinase was detected in ascophyllan-treated RAW264.7 cells. Our findings suggest for the first time that ascophyllan can stimulate macrophages to produce ROS through the activations of NADPH oxidase and JNK MAP kinase.
Keywords: Ascophyllan / Ascophyllum nodosum / NADPH oxidase / RAW264.7 cells / Reactive oxygen species / Respiratory burst
URI: http://hdl.handle.net/10069/31123
ISSN: 01418130
DOI: 10.1016/j.ijbiomac.2012.09.008
Rights: © 2012 Elsevier B.V. / NOTICE: this is the author’s version of a work that was accepted for publication in International Journal of Biological Macromolecules. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in International Journal of Biological Macromolecules, 52(2013)
Type: Journal Article
Text Version: author
Appears in Collections:Articles in academic journal

Citable URI : http://hdl.handle.net/10069/31123

All items in NAOSITE are protected by copyright, with all rights reserved.


Valid XHTML 1.0! Copyright © 2006-2015 Nagasaki University Library - Feedback Powerd by DSpace