DSpace university logo mark
Advanced Search
Japanese | English 

NAOSITE : Nagasaki University's Academic Output SITE > Faculty of Fisheries > Bulletin > Bulletin of the Faculty of Fisheries > 第29号 >


File Description SizeFormat
suisan29_109.pdf443.48 kBAdobe PDFView/Open

Title: 食品防腐剤フリルフラマイドのカラムクロマトグラフィーによるシス体,トランス体の分離定量
Other Titles: Separation and Determination of Cis- and Trans-Furylfuramide as Food Preservatives by Silicagel Column Chromatography
Authors: 石原, 忠 / 高本, 健一 / 保田, 正人
Authors (alternative): Ishihara, Tadashi / Komoto, Kenichi / Yasuda, Masato
Issue Date: Aug-1970
Publisher: 長崎大学水産学部
Citation: 長崎大学水産学部研究報告, v.29, pp.109-116; 1970
Abstract: A study was made on separation and determination of cis- and trans-furylfuramide which was extracted from kamaboko (fish cake). The procedures and the results can be summerized as follows. Cis- and trans-furylfuramide were separated by silicagel column chromatographic procedure using WAKOGEL C-200, which was soaked in 5 volumes of 20% acetic acid for 48 hours, then washed with boiling water 6 or 7 times, and after repeating the same treatment activated for 24 hours at 150℃, or which was refluxed with 5 volumes of 20% acetic acid on the free flame for 6 hours, then washed and activated. The silicagel was packed in column with n-hexan and the elution was carried out with ethyl ether. (Fig. 1) Cis- and trans-furylfuramide, separated through the column, were determined by ultraviolet spectroscopy at 380 mμ (trans-furylfuramide) and 390 mμ (cis-furylfuramide) in ethyl ether. Recovery rates of cis- and trans-furylfuramide during the column chromatography were 95~97%. (Tables 1, 2). The extract of kamaboko did not prevent the deter-mination of cis- and trans-furylfuramide. (Fig. 2, Table 2) / フリルフラマイドのcis体とtrans体のシリカゲルカラムクロマトグラフイーによる分離定量法について検討し次の結果を得た. 1. 分離用シリカゲルはWAKOGEL C-200を前処理として5倍量の20%酢酸で2日間の浸漬処理を2回行なうかまたは同量,同濃度の酢酸にて6時間還流する必要がある. 2. 上記のシリカゲルを150℃で24時間活性化し,n-ヘキサンでカラム(0.8×13cm)を調製しエチルエーテルにて毎分1mlの流速でクロマトグラフイーを行なえば両者を完全に分離できその回収率も高かった. 3. 煉製品の抽出液中の夾雑物はカラムクロマトグラフイーの溶出位置を少し遅くらせるが分離および回収率にはほとんど影響を与えなかった.
URI: http://hdl.handle.net/10069/31201
ISSN: 05471427
Type: Departmental Bulletin Paper
Text Version: publisher
Appears in Collections:第29号

Citable URI : http://hdl.handle.net/10069/31201

All items in NAOSITE are protected by copyright, with all rights reserved.


Valid XHTML 1.0! Copyright © 2006-2015 Nagasaki University Library - Feedback Powerd by DSpace