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タイトル: 凍結アサクサノリ糸状体の生存と殻胞子放出
その他のタイトル: Viability and Spore-liberation of Conchocelis-phase, Porphyra tenera, Freeze-preserved in Sea Water
著者: 右田, 清治
著者(別表記) : Migita, Seiji
発行日: 1967年 3月
出版者: 長崎大学水産学部
引用: 長崎大学水産学部研究報告, v.22, pp.33-43; 1967
抄録: There are distinct differences between thallus- and Conchocelisphases of Porphyra tenera KJELLMAN in general ecological aspects, especially in degree of drying injury. Though the thallus-phase of this alga shows high frost-resistance, it may be considered that the Conchocelis-phase is affected unfavorably by freezing and freeze-preservation. The present report deals with the frost-resistance of Conchocelis of Porphyra tenera, using the free- and shell-living materials. The liberation of conchospores from the shell-living Conchocelis freezepreserved at low temperatures was also observed. The results obtained are summarized as follos: When the Conchocelis-filaments were frozen and freeze-preserved in sea water, they showed lower frost-resistance than the leafy thalli. In the freezing of free- and shell-living Conchocelis-filaments, the former showed higher survival-rates of branches and conchosporangial cells. On the other hand, conchosporangial cells showed higher resistivity than cells of branches against freezing injury. Of those conchosporangial cells, mature divided sporangia survived well after freeze preservation at -20℃, and could tolerate without remarkable damage at least for 1 month. In the freezing at various cooling rates, ranging from 0.05℃/min to 40℃/min, the survival of Conchocelis-cells was less in rapid cooling than in slow cooling. The survival of Conchocelis-cells frozen at various low tempera-tures decreased according as temperature lowered. Furthermore, the survived cells of long preserved Conchocelis gradually decreased in number during freeze-preservation. Some shell-living Conchocelis, freeze-preserved for 1 month at -20℃ and then thawed, liberated numerous conchospores for the following several days. / アサクサノリの無基質糸状体,貝殼穿孔糸状体を海水中で凍結し,糸状体の枝や殼胞子嚢細胞の生存率をしらべた.また短期間凍結冷蔵した貝殼穿孔糸状体で解凍後の殼胞子放出数を測定した. 1. 凍結に対して,糸状体は葉体より抵抗性が低く,また貝殼穿孔糸状体は無基質糸状体に比べて凍害を受け易い. 2. 糸状体の殻胞子嚢細胞は枝より凍結に強く,とくに内容が分裂した成熟殻胞子嚢細胞は強い耐凍性を示した. 3. 冷却速度と生存率の関係は,急速凍結で凍死が多く緩慢凍結で生存が多い傾向がみられた. 4. 凍結温度が低くなるにつれて,糸状体の凍死細胞は増加する.また凍結冷蔵の期間が長くなると,生存率は徐々に低下する. 5. 貝殼穿孔糸状体を短期間(1ケ月以内)凍結冷蔵した場合は,解凍後多くの殼胞子を放出するものがみられた.
URI: http://hdl.handle.net/10069/31416
ISSN: 05471427
資料タイプ: Departmental Bulletin Paper
原稿種類: publisher

引用URI : http://hdl.handle.net/10069/31416



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