DSpace university logo mark
Advanced Search
Japanese | English 

NAOSITE : Nagasaki University's Academic Output SITE > School of Pharmaceutical Sciences > Articles in academic journal >

Microplate analytical method for quinones by pulse photo-irradiation and chemiluminescence detection

File Description SizeFormat
Analyst137_4802.pdf409.24 kBAdobe PDFView/Open

Title: Microplate analytical method for quinones by pulse photo-irradiation and chemiluminescence detection
Authors: Elgawish, Mohamed Saleh / Shimomai, Chikako / Kishikawa, Naoya / Ohyama, Kaname / Nakashima, Kenichiro / Kuroda, Naotaka
Issue Date: 21-Oct-2012
Publisher: Royal Society of Chemistry
Citation: Analyst, 137(20), pp.4802-4808; 2012
Abstract: Quinones are widely distributed in nature and have various bioactivities. Besides, quinones are also considered as toxicological intermediates which cause severe dangerous effects. Hereby, a sensitive, simple, and rapid method is reported for quinones determination. The proposed method employed time resolved fluorescence (TRF) microplate reader based chemiluminescent (CL) detection for the first time as a novel approach for measurement. Under pulse photo-irradiation, the unique photochemical characteristic of quinones is exploited to liberate reactive oxygen species (ROS) which reacted with photosensitized CL reagent. L-012, luminol analogue, was selected for its high sensitivity. Under our investigation, para-quinones showed high CL response when compared to ortho-quinones. A linear response was obtained for studied quinone concentrations in the range of 0.05-50 μM for 1,4-naphthquinone and of 0.05-150 μM for 2-methyl-1,4-naphthoquinone (menadione) and 9,10-anthraquinone with detection limit (blank + 3SD) of 0.01 μM. The proposed method allowed the rapid determination of large number of samples in very short time (96 sample/125 s). The proposed method was successfully applied for determination of menadione in spiked human serum.
Keywords: luminol / menadione / quinone derivative / reactive oxygen metabolite / article / blood / chemistry / human / luminescence / pH / Humans / Hydrogen-Ion Concentration / Luminescent Measurements / Luminol / Quinones / Reactive Oxygen Species / Vitamin K 3
URI: http://hdl.handle.net/10069/31463
ISSN: 00032654
DOI: 10.1039/c2an35353a
Rights: © 2012 The Royal Society of Chemistry.
Type: Journal Article
Text Version: author
Appears in Collections:Articles in academic journal

Citable URI : http://hdl.handle.net/10069/31463

All items in NAOSITE are protected by copyright, with all rights reserved.


Valid XHTML 1.0! Copyright © 2006-2015 Nagasaki University Library - Feedback Powerd by DSpace