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アマノリ葉体の生体凍結保存-2 : 冷却速度と葉体含水量の耐凍性に及ぼす影響


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Title: アマノリ葉体の生体凍結保存-2 : 冷却速度と葉体含水量の耐凍性に及ぼす影響
Other Titles: Freeze-Preservation of Porphyra Thalli in Viable State―II. Effect of Cooling Velocity and Water Content of Thalli on the Frost-Resistance
Authors: 右田, 清治
Authors (alternative): Migita, Seiji
Issue Date: Nov-1966
Publisher: 長崎大学水産学部
Citation: 長崎大学水産学部研究報告, v.21, pp.131-138; 1966
Abstract: From results of the previous work, it had been found that Porphyra thalli are highly resistant to low temperature, and that they can tolerate 4 months' freeze-preservation in half-dried state. In this paper, the author studied on the effect of cooling rate and of water content of thalli on the frost-resistance, through use of P. tenera, P. yezoensis and P. suborbiculata as materials. The surviving cells were determined under the microscope without any treatment. The results obtained are summarized as follows : 1. When the thalli were frozen in sea water at various cooling rates ranging from 0.02℃/min to 100℃/min and then thawed in room temperature, they showed high frost-resistance, but the survival rate of cells frozen in extremely rapid cooling (100℃/min) or slow cooling (0.02℃/min) more or less decreased. 2. In rapid freezing at temperatures ranging from -10℃ to 70℃ the cells of thalli could tolerate without any injury above -20℃, but surviving cells gradually decreased below -40℃. When deep-frozen at -75℃, the survival of thalli frozen in half-dried state were more than those frozen in sea water. 3. Materials frozen at 75℃ after pre-freezing at -20℃ for 24 hours showed higher values in cell survival than the other cases without pre-freezing. 4. The water content of thalli is closely correlated with the survival of thalli in freeze-preservation. Materials dried up to about 30 per cent water content showed high resistivity against freeze-injury, and some of them survived during one year's freeze-preservation at about -20℃. / アマノリ葉体の凍結保存において,冷却速度や葉体の含水量などの凍結条件か葉体細胞の耐凍性に及ほす影響について実験した. 1 アマノリ葉体を海水中で各種の冷却速度で凍結した場合,葉体細胞は002~100°C/minの広い速度範囲で凍結に対し強い抵抗性を示すが,1000C/minの急速凍結や002°C/minの緩慢凍結では生存率がやや低下した.2 -70°Cまでの段階的な温度で急速凍結すると,-30°Cまでは生存率はかなり高いが,それ以下の温度では低温になるほど生存細胞は少なくなる.しかし,半乾燥状態の凍結では海水中に比べてより多くの細胞が生き残った. 3 海水中の凍結において,-20°Cで24時間予備凍結したものは,その後-75°Cまで急速冷却しても生存率が高く,予備凍結の効果が認められた. 4.凍結前の葉体の含水量は耐凍性と深い関係があり,含水量20~40%で生存細胞が多く,それ以上の水分量でも過度に乾燥したものでも凍死細胞が増加する傾向がみられた.
URI: http://hdl.handle.net/10069/31479
ISSN: 05471427
Type: Departmental Bulletin Paper
Text Version: publisher
Appears in Collections:第21号

Citable URI : http://hdl.handle.net/10069/31479

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