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Crystallization and preliminary crystallographic study of oligomers of the haemolytic lectin CEL-III from the sea cucumber Cucumaria echinata


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Title: Crystallization and preliminary crystallographic study of oligomers of the haemolytic lectin CEL-III from the sea cucumber Cucumaria echinata
Authors: Unno, Hideaki / Hisamatsu, Keigo / Nagao, Tomonao / Tateya, Yuki / Matsumoto, Naoki / Goda, Shuichiro / Hatakeyama, Tomomitsu
Issue Date: Apr-2013
Publisher: International Union of Crystallography
Citation: Acta Crystallographica Section F Structural Biology and Crystallization Communications; 69(4), pp.416-420; 2013
Abstract: CEL-III is a Ca2+-dependent haemolytic lectin isolated from the marine invertebrate Cucumaria echinata. This lectin binds to Gal/GalNAc-containing carbohydrate chains on the cell surface and, after conformational changes, oligomerizes to form ion-permeable pores in cell membranes. CEL-III also forms soluble oligomers similar to those formed in cell membranes upon binding of specific carbohydrates in high-pH and high-salt solutions. These soluble and membrane CEL-III oligomers were crystallized and X-ray diffraction data were collected. Crystals of soluble oligomers and membrane oligomers diffracted X-rays to 3.3 and 4.2 Å resolution, respectively, using synchrotron radiation and the former was found to belong to space group C2. Self-rotation functional analysis of the soluble oligomer crystal suggested that it might be composed of heptameric CEL-III.
Keywords: Cucumaria echinata / haemolytic lectin CEL-III
URI: http://hdl.handle.net/10069/32218
DOI: 10.1107/S1744309113004065
Rights: © 2013 International Union of Crystallography
Type: Journal Article
Text Version: publisher
Appears in Collections:Articles in academic journal

Citable URI : http://hdl.handle.net/10069/32218

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