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第四アンモニウム塩基および関連化合物のイオン交換ペーパークロマトグラフィー(2)


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Title: 第四アンモニウム塩基および関連化合物のイオン交換ペーパークロマトグラフィー(2)
Other Titles: Rapid Separation and Identification of Quaternary Ammonium Bases and Related Compounds with Ion Exchange paper (2)
Authors: 竹友, 一成
Authors (alternative): Taketomo, Kazushige
Issue Date: 28-Feb-1974
Publisher: 長崎大学教育学部
Citation: 長崎大学教育学部自然科学研究報告. vol.25, p.69-78; 1974
Abstract: In order to apply ion exchange paper chromatography to forensic chemistry, quaternary ammonium bases and related compounds, in the form of reineckates, were examined whether they were chromatographically detected with ion exchange paper. The ion exchange paper, amberlite SA-2 was used. A dimethyl sulfoxide solution of the reineckate was spotted at the starting point that was at a 4cm distance from the bottom edge of the ion exchange paper strip. The strip was hung in a chamber at 30℃, the bottom edge of strip was immersed in lM-Na2HPO4-NaOH buffer solution (pH 13.30), and the ascending development was applied. After the run was completed, the strip was dried and passed through a freshly prepared solution of 2% phosphomolybdic acid in lM hydrochloric acid. The presence of the bases was evidenced by the appearance of the yellow spot. Under such a condition, Rf values of the bases were as follows: 0.44 for choline, 0.20 for neurine, 0.18 for tetraethylammonium, 0.79 for betaine alde- hyde, 0.74 for creatinine, and 0.39 for antipyrine. It was impossible to detect betaine, atropine, and trimethylamine oxide by the above method. Irradiation of light on a dimethyl sulfoxide solution of choline remeckate had effect on the rate of release of choline from choline reineckate. Also, other careful investigation of the release showed that the release was caused by amberlite SA-2 during the run. Beside, this method was applied to the detection of choline in the plants. / 有機塩基のライネッケートを試料とするイオン交換ぺ一パークロマトグラフィーを試み,次の結果を得た。(1)イオン交換紙としてアンバーライトSA-2を,展開液として1M-Na2HPO4-NaOH:緩衝液(pH15.50)を用いて,有機塩基のラィネッケートからもとの有機塩基を遊離再生せしめるための特別の操作を行なうことなく,有機塩基の分離,検出および定量を迅速かつ簡単に行なうことができた。(2)有機塩基の遊離再生に関与する因子は光エネルギーおよびアンバーライトSA-2であった。(3)本法をアキのノゲシおよびオニタビラコに適用し,それぞれコリンを迅速かつ簡単に検出することができた。
URI: http://hdl.handle.net/10069/32966
ISSN: 0386443X
Type: Departmental Bulletin Paper
Text Version: publisher
Appears in Collections:No. 25

Citable URI : http://hdl.handle.net/10069/32966

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