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Title: イオン交換紙による第四アンモニウム塩基および関連化合物の迅速な分離検出〔イオン交換ペーパークロマトグラフィーの裁判化学への応用-3-〕
Other Titles: Rapid Separation and Identification of Quaternary Ammonium Bases and Related Compounds with Ion Exchange Paper
Authors: 竹友, 一成
Authors (alternative): Taketomo, Kazushige
Issue Date: 28-Feb-1971
Publisher: 長崎大学教育学部
Citation: 長崎大学教育学部自然科学研究報告. vol.22, p.53-63; 1971
Abstract: In order to apply ion exchange paper chromatography to forensic chemistry, quaternary ammonium bases and related compounds, in the form of penodides, were examined whether they were chromatographically detected with the ion exchange paper. The ion exchange paper, amberlite SA-2 was used. A ethyl alcohol (or acetone) solution of the periodides was spotted at the starting point that was at a 4 cm distance from the bottom edge of the ion exchange paper strip. The strip was hung in a chamber at 30℃, the bottom edge of the strip was immersed in 1M Na2HPO4-NaOH buffer solution (pH 13.30), and the ascending development was applied. During the run, stainish brown colour of the periodide was faded away. After the run was completed, the strip was dried and passed through a freshly prepared solution of 2% phosphomolybdic acid in 1 N hydrochloric acid. The presence of quaternary ammonium bases and related compounds was evidenced by the appearance of the yellow spot. Under such a condition, Rf values of quaternary ammoniun bases and related compounds were as follows: 0.44 for choline chloride, 0.20 for neurine chloride, 0.18 for tetraethylammonium chloride, 0.00 for cetyltrimethylammonium bromide, 0.34 for caffeine, 0.11 (0.40) for antipyrine, 0.01 for ephedrine hydrochloride, and 0.74 for creatinine. It was impossible to detect betaine and atropine sulfate by the above method. It is presumed that the fading of the stainish brown periodide, during the run, may be mainly due to the following mechanism: (organic base) Ix + x NaOH→organic base + x/2 NaI + x/2 NaIO + x/2 H2O Besides, this method was applied to the detection of choline in human urine, human semen, human internal organs, and rabbit internal organs.
URI: http://hdl.handle.net/10069/33056
ISSN: 0386443X
Type: Departmental Bulletin Paper
Text Version: publisher
Appears in Collections:No. 22

Citable URI : http://hdl.handle.net/10069/33056

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