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A novel lophine-based fluorescence probe and its binding to human serum albumin


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Title: A novel lophine-based fluorescence probe and its binding to human serum albumin
Authors: Kishikawa, Naoya / Ohyama, Kaname / Saiki, Akane / Matsuo, Aya / Ali, Marwa Fathy Bakr / Wada, Mitsuhiro / Nakashima, Kenichiro / Kuroda, Naotaka
Issue Date: 30-May-2013
Publisher: Elsevier
Citation: Analytica Chimica Acta, 780, pp.1-6; 2013
Abstract: The binding of a lophine-based fluorescence probe, 4-[4-(4-dimethylaminophenyl)-5-phenyl-1H-imidazol-2-yl]benzoic acid methyl ester (DAPIM) with human serum albumin (HSA) was investigated by fluorescence spectroscopy under physiological conditions. While DAPIM shows extreme low fluorescence in aqueous solution, DAPIM binding with HSA emits strong fluorescence at 510nm. The binding constant and binding number determined by Scatchard plot was 3.65×106M-1 and 1.07, respectively. Competitive binding between DAPIM and other ligands such as warfarin, valproic acid, diazepam and oleic acid, were also studied fluorometrically. The results indicated that the primary binding site of DAPIM to HSA is site II at subdomain IIIA. DAPIM can be a useful fluorescence probe for the characterization of drug-binding sites. In addition to the interaction study, because the fluorescence intensity of DAPIM increased in proportion to HSA concentration, its potential in HSA assay for serum sample was also evaluated.
Keywords: 4-[4-(4-Dimethylaminophenyl)-5-phenyl-1H-imidazol-2-yl]benzoic acid methyl ester / Binding constant / Binding site / Human serum albumin
URI: http://hdl.handle.net/10069/33128
ISSN: 00032670
DOI: 10.1016/j.aca.2013.04.003
Rights: © 2013 Published by Elsevier B.V. / NOTICE: this is the author’s version of a work that was accepted for publication in Analytica Chimica Acta. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Analytica Chimica Acta, 780(2013)
Type: Journal Article
Text Version: author
Appears in Collections:Articles in academic journal

Citable URI : http://hdl.handle.net/10069/33128

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