DSpace university logo mark
Advanced Search
Japanese | English 

NAOSITE : Nagasaki University's Academic Output SITE > School of Pharmaceutical Sciences > Articles in academic journal >

A simple and rapid chemiluminescence assay for on-site analysis of paraquat using a portable luminometer

File Description SizeFormat
ForTox31_301.pdf1.29 MBAdobe PDFView/Open

Title: A simple and rapid chemiluminescence assay for on-site analysis of paraquat using a portable luminometer
Authors: Kishikawa, Naoya / Higuchi, Sho / Ohyama, Kaname / Nakashima, Kenichiro / Kuroda, Naotaka
Issue Date: Jul-2013
Publisher: 日本法中毒学会 / Japanese Association of Forensic Toxicology
Citation: Forensic Toxicology, 31(2), pp.301-306; 2013
Abstract: Paraquat (N,N′-dimethyl-4,4′-bipyridinium dichloride) is one of the most widely used herbicides owing to its high efficacy and low environmental persistence. However, because paraquat has significant acute toxicity, fatalities are often caused by accidental or voluntary ingestion of paraquat. In consideration of the strong toxicity and fast-Acting property of paraquat, on-site analysis at accident scenes should be effective in facilitating immediate medical treatment. In this study, a simple and rapid chemiluminescence assay using a portable luminometer was developed for on-site analysis of paraquat. The proposed assay is based on luminol chemiluminescence detection of superoxide anion radical resulting from the redox reaction between paraquat and dithiothreitol. Intense chemiluminescence was observed after mixing of paraquat and dithiothreitol in the presence of luminol. Because the chemiluminescence intensity was proportional to the concentration of paraquat, a quantitative measurement of paraquat was possible. The calibration curve for standard paraquat solution was linear from 0.025 to 2.5 μM with the correlation coefficient of 0.992; the detection limit (blank + 3SD) was 22 nM. The proposed assay was applied to determine paraquat in beverage samples after a cation exchange clean-up procedure. Given that the portable luminometer used in this study is small and lightweight, the proposed assay should be useful for on-site analysis of paraquat.
Keywords: Beverage samples / Chemiluminescence / On-site analysis / Paraquat / Portable luminometer / Redox cycle
URI: http://hdl.handle.net/10069/33435
ISSN: 18608965
DOI: 10.1007/s11419-012-0175-0
Rights: © 2013 Japanese Association of Forensic Toxicology and Springer Japan. / The final publication is available at link.springer.com.
Type: Journal Article
Text Version: author
Appears in Collections:Articles in academic journal

Citable URI : http://hdl.handle.net/10069/33435

All items in NAOSITE are protected by copyright, with all rights reserved.


Valid XHTML 1.0! Copyright © 2006-2015 Nagasaki University Library - Feedback Powerd by DSpace