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Immune Complexome Analysis


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Title: Immune Complexome Analysis
Authors: Ohyama, Kaname / Kuroda, Naotaka
Issue Date: 2013
Publisher: Academic Press Inc.
Citation: Advances in Clinical Chemistry, 60, pp.129-141; 2013
Abstract: Immune complexes (ICs) are produced during an immune response and may reflect some aspects of an ongoing immune response. Therefore, the identity of antigens incorporated into ICs provides the information that in the future may aid in the development of diagnosis and treatment strategies for autoimmune diseases, infection, cancer, and transplantation therapy, and this information might be more relevant than the information on free antigens. Because ICs may contain many antigens, comprehensive identification and profiling of such antigens are more effective than immunoblotting detection. Here, we introduced mass spectrometry (MS)-based two approaches (immunoproteomics and immune complexome analysis) to comprehensively identify the antigens. Immunoproteomics is a concept to identify disease-associated antigens that elicit immune responses by combining protein separation (two-dimensional electrophoresis, gel-free separation), immunological detection (Western blotting), and MS or by combining immunocapture and MS. Immune complexome analysis is designed for identifying antigens in circulating ICs and consists of ICs separation from serum and direct tryptic digestion followed by nano-liquid chromatography-tandem MS.
Keywords: Antigen profiling / Immune complexome analysis / Immunoproteomics / Mass spectrometry
URI: http://hdl.handle.net/10069/33454
ISSN: 00652423
DOI: 10.1016/B978-0-12-407681-5.00004-0
Rights: © 2013 Elsevier Inc. / NOTICE: this is the author’s version of a work that was accepted for publication in Advances in Clinical Chemistry. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Advances in Clinical Chemistry, 60(2013)
Type: Book
Text Version: author
Appears in Collections:Articles in academic journal

Citable URI : http://hdl.handle.net/10069/33454

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