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Chromatographic determination of aliphatic aldehydes in human serum after pre-column derivatization using 2,2′-furil, a novel fluorogenic reagent

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Title: Chromatographic determination of aliphatic aldehydes in human serum after pre-column derivatization using 2,2′-furil, a novel fluorogenic reagent
Authors: Fathy Bakr Ali, Marwa / Kishikawa, Naoya / Ohyama, Kaname / Abdel-Mageed Mohamed, Horria / Mohamed Abdel-Wadood, Hanaa / Mohamed Mohamed, Ashraf / Kuroda, Naotaka
Issue Date: 26-Jul-2013
Publisher: Elsevier
Citation: Journal of Chromatography A, 1300, pp.199-203; 2013
Abstract: A novel, highly sensitive and selective fluorimetric liquid chromatographic method for simultaneous determination of medium chain aliphatic aldehydes was developed. The method was based on the derivatization of aliphatic aldehydes with 1,2-di(2-furyl)-1,2-ethanedione (2,2'-furil), a novel fluorogenic reagent, to form highly fluorescent difurylimidazole derivatives. The fluorescence derivatives were separated in less than 20. min on a reversed-phase ODS column using an isocratic elution with a mixture of methanol-water (80:20, v/v%). The detection limits were from 0.19 to 0.50. nM (1-10. fmol/injection) at a signal-to-noise ratio (S/N) of 3. This method was successfully applied for monitoring of aliphatic aldehydes in healthy human sera by a simple pretreatment procedure without interferences from serum constituents.
Keywords: 1,2-Di(2-furyl)-1,2-ethanedione (2,2'-furil) / Aliphatic aldehydes (lipid peroxidation) / Difurylimidazole derivatives / Pre-column fluorogenic derivatization / Serum analysis
URI: http://hdl.handle.net/10069/33615
ISSN: 00219673
DOI: 10.1016/j.chroma.2013.03.033
Rights: © 2013 Elsevier B.V. / NOTICE: this is the author’s version of a work that was accepted for publication in Journal of Chromatography A. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Journal of Chromatography A, 1300(2013)
Type: Journal Article
Text Version: author
Appears in Collections:Articles in academic journal

Citable URI : http://hdl.handle.net/10069/33615

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