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Determination of the ratio between mercaptalbumin and nonmercaptalbumin by HPLC with fluorescence probe specifically binding to albumin


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Title: Determination of the ratio between mercaptalbumin and nonmercaptalbumin by HPLC with fluorescence probe specifically binding to albumin
Authors: Ohyama, Kaname / Kishikawa, Naoya / Matsuo, Aya / Imazato, Takahiro / Ueki, Yukitaka / Wada, Mitsuhiro / Nakashima, Kenichiro / Kuroda, Naotaka
Issue Date: 25-Jan-2014
Publisher: Elsevier
Citation: Journal of Pharmaceutical and Biomedical Analysis, 88, pp.170-173; 2014
Abstract: A simple and selective HPLC-fluorescence (FL) method with FL probe, 4-[4-(4-dimethylaminophenyl)-5-phenyl-1. H-imidazol-2-yl]benzoic acid methyl ester (DAPIM), for simultaneous determination of mercaptalbumin (HMA) and nonmercaptalbumin (HNA1) was developed. After HMA and HNA1 were separated on an ion-exchange column, they were on-line and post-column mixed with DAPIM. The DAPIM-albumin complex produces FL (λex 370. nm and λem 510. nm); however, DAPIM solution never gives the FL. Based on this mechanism, selective determination of HMA and HNA1 were achieved without any pretreatment and interfering peak. The proposed method was applied to the measurement of HMA and HNA1 in human serum of healthy volunteers and diabetes mellitus patients.
Keywords: 4-[4-(4-dimethylaminophenyl)-5-phenyl-1H-imidazol-2-yl]Benzoic acid methyl ester / Fluorescence probe / Mercaptalbumin / Nonmercaptalbumin / Redox state
URI: http://hdl.handle.net/10069/33940
ISSN: 07317085
DOI: 10.1016/j.jpba.2013.08.009
Rights: © 2013 Elsevier B.V.
Type: Journal Article
Text Version: author
Appears in Collections:Articles in academic journal

Citable URI : http://hdl.handle.net/10069/33940

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