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Efficacy of AiiM, an N-Acylhomoserine Lactonase, against Pseudomonas aeruginosa in a Mouse Model of Acute Pneumonia

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Title: Efficacy of AiiM, an N-Acylhomoserine Lactonase, against Pseudomonas aeruginosa in a Mouse Model of Acute Pneumonia
Authors: Migiyama, Yohei / Kaneko, Yukihiro / Yanagihara, Katsunori / Morohoshi, Tomohiro / Morinaga, Yoshitomo / Nakamura, Shigeki / Miyazaki, Taiga / Hasegawa, Hiroo / Izumikawa, Koichi / Kakeya, Hiroshi / Kohrogi, Hirotsugu / Kohno, Shigeru
Issue Date: Aug-2013
Publisher: American Society for Microbiology
Citation: Antimicrobial Agents and Chemotherapy, 57(8), pp.3653-3658; 2013
Abstract: Quorum sensing (QS) in Pseudomonas aeruginosa regulates the production of many virulence factors and plays an important role in the pathogenesis of P. aeruginosa infection. N-acyl homoserine lactones (AHL) are major QS signal molecules. Recently, a novel AHL-lactonase enzyme, AiiM, has been identified. The aim of this study was to evaluate the effect of AiiM on the virulence of P. aeruginosa in a mouse model of acute pneumonia. We developed a P. aeruginosa PAO1 strain harboring an AiiM-expressing plasmid. The production of several virulence factors by the AiiM-expressing strain was examined. Mice were intratracheally infected with an AiiM-expressing PAO1 strain. Lung histopathology, bacterial burden, and bronchoalveolar lavage (BAL) fluid were assessed at 24 h postinfection. AiiM expression in PAO1 reduced production of AHL-mediated virulence factors and attenuated cytotoxicity against human lung epithelial cells. In a mouse model of acute pneumonia, AiiM expression reduced lung injury and greatly improved the survival rates. The levels of proinflammatory cytokines and myeloperoxidase activity in BAL fluid were significantly lower in mice infected with AiiM-expressing PAO1. Thus, AiiM can strongly attenuate P. aeruginosa virulence in a mammalian model and is a potential candidate for use as a therapeutic agent against P. aeruginosa infection.
Keywords: AiiM / mice / pneumonia / Pseudomonas aeruginosa / quorum sensing
URI: http://hdl.handle.net/10069/34031
ISSN: 00664804
DOI: 10.1128/AAC.00456-13
Rights: © 2013, American Society for Microbiology. All Rights Reserved.
Type: Journal Article
Text Version: author
Appears in Collections:Articles in academic journal

Citable URI : http://hdl.handle.net/10069/34031

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