DSpace university logo mark
Advanced Search
Japanese | English 

NAOSITE : Nagasaki University's Academic Output SITE > School of Dentistry > Articles in academic journal >

A bone substitute with high affinity for vitamin D-binding protein―relationship with niche of osteoclasts

File Description SizeFormat
JCMM18_170.pdf926.42 kBAdobe PDFView/Open

Title: A bone substitute with high affinity for vitamin D-binding protein―relationship with niche of osteoclasts
Authors: Ikeda, Tohru / Kasai, Michiyuki / Tatsukawa, Eri / Kamitakahara, Masanobu / Shibata, Yasuaki / Yokoi, Taishi / Nemoto, Takayuki K. / Ioku, Koji
Issue Date: Jan-2014
Publisher: Blackwell Publishing
Citation: Journal of Cellular and Molecular Medicine, 18(1), pp.170-180; 2014
Abstract: The biological activity of osteoblasts and osteoclasts is regulated not only by hormones but also by local growth factors, which are expressed in neighbouring cells or included in bone matrix. Previously, we developed hydroxyapatite (HA) composed of rod-shaped particles using applied hydrothermal methods (HHA), and it revealed mild biodegradability and potent osteoclast homing activity. Here, we compared serum proteins adsorbed to HHA with those adsorbed to conventional HA composed of globular-shaped particles (CHA). The two ceramics adsorbed serum albumin and γ-globulin to similar extents, but affinity for γ-globulin was much greater than that to serum albumin. The chemotactic activity for macrophages of serum proteins adsorbed to HHA was significantly higher than that of serum proteins adsorbed to CHA. Quantitative proteomic analysis of adsorbed serum proteins revealed preferential binding of vitamin D-binding protein (DBP) and complements C3 and C4B with HHA. When implanted with the femur of 8-week-old rats, HHA contained significantly larger amount of DBP than CHA. The biological activity of DBP was analysed and it was found that the chemotactic activity for macrophages was weak. However, DBP-macrophage activating factor, which is generated by the digestion of sugar chains of DBP, stimulated osteoclastogenesis. These results confirm that the microstructure of hydroxyapatite largely affects the affinity for serum proteins, and suggest that DBP preferentially adsorbed to HA composed of rod-shaped particles influences its potent osteoclast homing activity and local bone metabolism.
Keywords: Hydroxyapatite / Macrophage activating factor / Osteoclast / Serum proteins / Vitamin D-binding protein
URI: http://hdl.handle.net/10069/34156
ISSN: 15821838
DOI: 10.1111/jcmm.12180
Rights: © 2013 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine. / This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Type: Journal Article
Text Version: publisher
Appears in Collections:Articles in academic journal

Citable URI : http://hdl.handle.net/10069/34156

All items in NAOSITE are protected by copyright, with all rights reserved.


Valid XHTML 1.0! Copyright © 2006-2015 Nagasaki University Library - Feedback Powerd by DSpace