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Intratracheal Administration of Recombinant Human Keratinocyte Growth Factor Promotes Alveolar Epithelial Cell Proliferation during Compensatory Lung Growth in Rat


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Title: Intratracheal Administration of Recombinant Human Keratinocyte Growth Factor Promotes Alveolar Epithelial Cell Proliferation during Compensatory Lung Growth in Rat
Authors: Furukawa, Katsuro / Matsumoto, Keitaro / Nagayasu, Takeshi / Yamamoto-Fukuda, Tomomi / Tobinaga, Shuichi / Abo, Takafumi / Yamasaki, Naoya / Tsuchiya, Tomoshi / Miyazaki, Takuro / Kamohara, Ryotaro / Nanashima, Atsushi / Obatake, Masayuki / Koji, Takehiko
Issue Date: 28-Dec-2013
Publisher: 日本組織細胞化学会 / Japan Society of Histochemistry and Cytochemistry
Citation: ACTA HISTOCHEMICA ET CYTOCHEMICA, 46(6), pp.179-185; 2013
Abstract: Keratinocyte growth factor (KGF) is considered to be one of the most important mitogens for lung epithelial cells. The objectives of this study were to confirm the effectiveness of intratracheal injection of recombinant human KGF (rhKGF) during compensatory lung growth and to optimize the instillation protocol. Here, trilobectomy in adult rat was performed, followed by intratracheal rhKGF instillation with low (0.4 mg/kg) and high (4 mg/kg) doses at various time-points. The proliferation of alveolar cells was assessed by the immunostaining for proliferating cell nuclear antigen (PCNA) in the residual lung. We also investigated other immunohistochemical parameters such as KGF, KGF receptor and surfactant protein A as well as terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling. Consequently, intratracheal single injection of rhKGF in high dose group significantly increased PCNA labeling index (LI) of alveolar cells in the remaining lung. Surprisingly, there was no difference in PCNA LI between low and high doses of rhKGF with daily injection, and PCNA LI reached a plateau level with 2 days-consecutive administration (about 60%). Our results indicate that even at low dose, daily intratracheal injection is effective to maintain high proliferative states during the early phase of compensatory lung growth.
Keywords: Compensatory growth / Intratracheal injection / PCNA / Recombinant human KGF / Trilobectomy
URI: http://hdl.handle.net/10069/34164
ISSN: 00445991
DOI: 10.1267/ahc.13036
Rights: © 2013 The Japan Society of Histochemistry and Cytochemistry
Type: Journal Article
Text Version: none
Appears in Collections:Articles in academic journal

Citable URI : http://hdl.handle.net/10069/34164

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