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RELEVANCE OF MOLECULAR TESTS FOR HTLV-1 INFECTION AS CONFIRMATORY TESTS AFTER THE FIRST SERO-SCREENING


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Title: RELEVANCE OF MOLECULAR TESTS FOR HTLV-1 INFECTION AS CONFIRMATORY TESTS AFTER THE FIRST SERO-SCREENING
Authors: Ishihara, Kaori / Inokuchi, Naoko / Tsushima, Yuko / Tsuruda, Kazuto / Morinaga, Yoshitomo / Hasegawa, Hiroo / Yanagihara, Katsunori / Kamihira, Shimeru
Issue Date: 2-Jan-2014
Publisher: Taylor and Francis Inc.
Citation: Journal of Immunoassay and Immunochemistry, 35(1), pp.74-82; 2014
Abstract: The diagnosis of human T-cell leukemia virus type-1 (HTLV-1) infection has been widely examined by serologics. In the first screening tests, serological false negative and positive samples have been reduced thanks to advances in assay techniques that apply new emission agents and sensors. On the other hand, western blot (WB) remains problematic. For example, WB analysis yields many samples equivalent to antibody positive ones. To reduce the need for WB, an alternative testing strategy is required to detect HTLV-1 infection. Polymerase chain reaction (PCR) for the HTLV-1 provirus has recently been recommended for a final diagnosis of infection. However, although PCR is thought to be one element, the validation of detection performance for HTLV-1 infection between serological and molecular testing is not always clear. Thus, this study aimed to evaluate the accuracy and test the validity of an improved methodology for serological detection of HTLV-infection, as well as that of PCR. In conclusion, the high values of kappa-statistics are expected to deliver high quality in chemiluminescent enzyme immunoassay (or chemiluminescent immunoassay), while the problems with WB assays remain to be elucidated. As an alternative to WB, a combination of real-time qPCR and nested PCR is proposed as a suitable confirmatory test.
Keywords: CLEIA / CLIA / HTLV-1 / PCR / westernblot
URI: http://hdl.handle.net/10069/34177
ISSN: 15321819
DOI: 10.1080/15321819.2013.792832
Rights: This is an Author's Accepted Manuscript of an article published in Journal of Immunoassay and Immunochemistry, 35(1), pp.74-82; 2014 © Taylor & Francis, available online at: http://www.tandfonline.com/10.1080/15321819.2013.79283
Type: Journal Article
Text Version: author
Appears in Collections:Articles in academic journal

Citable URI : http://hdl.handle.net/10069/34177

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