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Development of anionic bubble lipopolyplexes for efficient and safe gene transfection with ultrasound exposure in mice

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Title: Development of anionic bubble lipopolyplexes for efficient and safe gene transfection with ultrasound exposure in mice
Authors: Kurosaki, Tomoaki / Kawakami, Shigeru / Higuchi, Yuriko / Suzuki, Ryo / Maruyama, Kazuo / Sasaki, Hitoshi / Yamashita, Fumiyoshi / Hashida, Mitsuru
Issue Date: 28-Feb-2014
Publisher: Elsevier
Citation: Journal of Controlled Release, 176, pp.24-34; 2014
Abstract: Anionic bubble lipopolyplexes have been developed as anionic ultrasound (US)-responsive gene delivery carriers with biocompatible compounds for efficient and safe transfection in mice. The particles of the anionic bubble lipopolyplexes were approximately 450-600 nm with an anionic surface charge. In the absence of US exposure, the bubble lipopolyplexes showed extremely low gene expression in the human vascular endothelial cell line EAhy926. The anionic bubble lipopolyplexes, however, delivered pDNA into cells without endocytosis and showed markedly high gene expression following US exposure. The anionic bubble lipopolyplexes showed little cytotoxicity in EAhy926 cells and little aggregation with erythrocytes. Following intravenous administration into mice, the anionic bubble lipopolyplexes showed high levels of gene expression in the liver, kidney, and spleen only after US exposure to the abdominal area. The level of gene expression in liver non-parenchymal cells was significantly higher than that in parenchymal cells. In addition, the anionic bubble lipopolyplexes did not show any severe hepatic toxicity and did not enhance the production of proinflammatory cytokines. Overall, we have succeeded in preparing anionic bubble lipopolyplexes for efficient and safe transfection with US exposure in mice.
Keywords: Biocompatibility / DNA / Liposomes / Self assembly / Sonoporation / Transfection
URI: http://hdl.handle.net/10069/34194
ISSN: 01683659
DOI: 10.1016/j.jconrel.2013.12.023
Rights: © 2013 Elsevier B.V. / NOTICE: this is the author’s version of a work that was accepted for publication in Journal of Controlled Release. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Journal of Controlled Release, 176, 1, (2014)
Type: Journal Article
Text Version: author
Appears in Collections:Articles in academic journal

Citable URI : http://hdl.handle.net/10069/34194

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