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Occult microscopic endometriosis: undetectable by laparoscopy in normal peritoneum


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Title: Occult microscopic endometriosis: undetectable by laparoscopy in normal peritoneum
Authors: Khan, Khaleque Newaz / Fujishita, Akira / Kitajima, Michio / Hiraki, Koichi / Nakashima, Masahiro / Masuzaki, Hideaki
Issue Date: Mar-2014
Publisher: Oxford University Press
Citation: Human Reproduction, 29(3), pp.462-472; 2014
Abstract: STUDY QUESTIONIs there any occurrence of hidden (occult) endometriotic lesions in normal peritoneum of women with and without visible endometriosis?SUMMARY ANSWERWe detected a slightly higher occurrence of occult microscopic endometriosis (OME) in normal peritoneum of women with visible endometriosis than in control women.WHAT IS KNOWN ALREADYBased on a small number of cases, the concept of invisible microscopic endometriosis in visually normal peritoneum has been reported for more than a decade but there is controversy regarding their tissue activity and clinical significance.STUDY DESIGN, SIZE, DURATIONThis case-controlled research study was conducted with prospectively collected normal peritoneal samples from 151 women with and 62 women without visible endometriosis.PARTICIPANTS/MATERIALS, SETTING, METHODSNormal peritoneal biopsy specimens from different pelvic sites of were collected during laparoscopy. A histological search of all peritoneal biopsy specimens for the detection of invisible endometriosis was done by immunoreaction to Ber-EP4 (epithelial cell marker), CD10 (stromal cell marker) and Calretinin (mesothelial cell marker). Tissue expression of estrogen/progesterone receptors (ER/PR) and cell proliferation marker, Ki-67, was performed by immunohistochemistry to identify tissue activity.MAIN RESULTS AND THE ROLE OF CHANCEThree different patterns of OME were detected based on (I) the presence of typical gland/stroma, (II) reactive hyperplastic change of endometrioid epithelial cells with surrounding stroma and (III) single-layered epithelium-lined cystic lesions with surrounding stroma. A higher tendency toward the occurrence of OME was found in women with visible endometriosis (15.2%, 23/151) compared with control women (6.4%, 4/62) (P = 0.06, χ2 test). The epithelial cells and/or stromal cells of OME lesions were immunoreactive to Ber-EP4 and CD10 but not reactive to Calretinin. ER and PR expression was observed in all patterns of OME lesions. Ki-67 index was significantly higher in pattern I/II OME lesions than in pattern III OME lesions (P< 0.05 for each).LIMITATIONS, REASONS FOR CAUTIONBias in the incidence rate of OME lesions in this study cannot be ignored, because we could not analyze biopsy specimens from the Pouch of Douglas of women with revised classification of the American Society of Reproductive Medicine Stage III-IV endometriosis due to the presence of adhesions in the pelvis.WIDER IMPLICATIONS OF THE FINDINGSWe re-confirmed a decade long old concept of invisible (occult) endometriosis in visually normal peritoneum of women with visible endometriosis. The existence of a variable amount of tissue activity in these occult lesions may contribute to the recurrence/occurrence of endometriosis or persistence/recurrence of pain manifestation in women even after successful ablation or excision of visible lesions by laparoscopy.
Keywords: estrogen receptor / Ki-67 / occult endometriosis / progesterone receptor / visible endometriosis
URI: http://hdl.handle.net/10069/34362
ISSN: 02681161
DOI: 10.1093/humrep/det438
Rights: © The Author 2013. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. / This is a pre-copy-editing, author-produced PDF of an article accepted for publication in Human Reproduction following peer review. The definitive publisher-authenticated version Human Reproduction, 29(3), pp.462-472; 2014 is available online at: http://dx.doi.org/10.1093/humrep/det438
Type: Journal Article
Text Version: author
Appears in Collections:Articles in academic journal

Citable URI : http://hdl.handle.net/10069/34362

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