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Liver Suction-Mediated Transfection in Mice Using a Pressure-Controlled Computer System


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Title: Liver Suction-Mediated Transfection in Mice Using a Pressure-Controlled Computer System
Authors: Shimizu, Kazunori / Zhang, Guangyuan / Kawakami, Shigeru / Taniguchi, Yota / Hayashi, Kouji / Hashida, Mitsuru / Konishi, Satoshi
Issue Date: 1-Apr-2014
Publisher: 日本薬学会 / Pharmaceutical Society of Japan
Citation: Biological and Pharmaceutical Bulletin, 37(4), pp.569-575; 2014
Abstract: We previously developed an in vivo tissue suction-mediated transfection method (denoted as the tissue suction method) for naked nucleic acids, such as plasmid DNA (pDNA) and small interfering RNA (siRNA), in mice. However, it remains unclear whether the suction pressure conditions affect the results of this method. Therefore, in the present study, we assembled a computer system to control the suction pressure and investigate the effects of the suction pressure conditions on the efficiency of the liver suction transfection of naked pDNA that encodes luciferase in mice. Using the developed system, we examined the effects of the minimum magnitude of the suction pressure, suction pressure waveform, and suction times of the luciferase expression level in mice livers. We determined that the liver suction method at -5 kPa was not only effective but also caused the lowest hepatic toxicity in mice. Additionally, the results indicated that the suction pressure waveform affects the luciferase expression levels, and a single period of suction on the targeted portion of the liver is sufficient for transfection. Thus, the developed system is useful for performing the tissue suction method with high accuracy and safety.
Keywords: Computer system / Gene delivery / Mechanical stimulus / Naked DNA / Tissue suction
URI: http://hdl.handle.net/10069/34435
ISSN: 09186158
DOI: 10.1248/bpb.b13-00776
Rights: © 2014 The Pharmaceutical Society of Japan.
Type: Journal Article
Text Version: publisher
Appears in Collections:Articles in academic journal

Citable URI : http://hdl.handle.net/10069/34435

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