DSpace university logo mark
Advanced Search
Japanese | English 

NAOSITE : Nagasaki University's Academic Output SITE > School of Medicine > Articles in academic journal >

Maintenance of stereocilia and apical junctional complexes by Cdc42 in cochlear hair cells


File Description SizeFormat
JCS127_2040.pdf6.84 MBAdobe PDFView/Open

Title: Maintenance of stereocilia and apical junctional complexes by Cdc42 in cochlear hair cells
Authors: Ueyama, Takehiko / Sakaguchi, Hirofumi / Nakamura, Takashi / Goto, Akihiro / Morioka, Shigefumi / Shimizu, Aya / Nakao, Kazuki / Hishikawa, Yoshitaka / Ninoyu, Yuzuru / Kassai, Hidetoshi / Suetsugu, Shiro / Koji, Takehiko / Fritzsch, Bernd / Yonemura, Shigenobu / Hisa, Yasuo / Matsuda, Michiyuki / Aiba, Atsu / Saito, Naoaki
Issue Date: May-2014
Publisher: Company of Biologists Ltd
Citation: Journal of Cell Science, 127(9), pp.2040-2052; 2014
Abstract: Cdc42 is a key regulator of dynamic actin organization. However, little is known about how Cdc42-dependent actin regulation influences steady-state actin structures in differentiated epithelia. We employed inner ear hair-cell-specific conditional knockout to analyze the role of Cdc42 in hair cells possessing highly elaborate stable actin protrusions (stereocilia). Hair cells of Atoh1-Cre;Cdc42flox/flox mice developed normally but progressively degenerated after maturation, resulting in progressive hearing loss particularly at high frequencies. Cochlear hair cell degeneration was more robust in inner hair cells than in outer hair cells, and began as stereocilia fusion and depletion, accompanied by a thinning and waving circumferential actin belt at apical junctional complexes (AJCs). Adenovirus-encoded GFP-Cdc42 expression in hair cells and fluorescence resonance energy transfer (FRET) imaging of hair cells from transgenicmice expressing a Cdc42-FRET biosensor indicated Cdc42 presence and activation at stereociliary membranes and AJCs in cochlear hair cells. Cdc42-knockdown in MDCK cells produced phenotypes similar to those of Cdc42-deleted hair cells, including abnormal microvilli and disrupted AJCs, and downregulated actin turnover represented by enhanced levels of phosphorylated cofilin. Thus, Cdc42 influenced the maintenance of stable actin structures through elaborate tuning of actin turnover, and maintained function and viability of cochlear hair cells.
Keywords: Actin turnover / Apical junctional complex / Cdc42 / Deafness / Fret / Hair cell / Stereocilia
URI: http://hdl.handle.net/10069/34499
ISSN: 00219533
DOI: 10.1242/jcs.143602
Rights: © 2014. Published by The Company of Biologists Ltd / This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
Type: Journal Article
Text Version: publisher
Appears in Collections:Articles in academic journal

Citable URI : http://hdl.handle.net/10069/34499

All items in NAOSITE are protected by copyright, with all rights reserved.

 

Valid XHTML 1.0! Copyright © 2006-2015 Nagasaki University Library - Feedback Powerd by DSpace