DSpace university logo mark
Japanese | English 

NAOSITE : Nagasaki University's Academic Output SITE > 030 医学部 > 030 学術雑誌論文 >

Differential infiltration of macrophages and prostaglandin production by different uterine leiomyomas

ファイル 記述 サイズフォーマット
HumRep21_2545.pdf1.54 MBAdobe PDF本文ファイル

タイトル: Differential infiltration of macrophages and prostaglandin production by different uterine leiomyomas
著者: Miura, Seiyou / Khan, Khaleque Newaz / Kitajima, Michio / Hiraki, Koichi / Moriyama, Shingo / Masuzaki, Hideaki / Samejima, Tetsurou / Fujishita, Akira / Ishimaru, Tadayuki
発行日: 2006年10月
出版者: Oxford University Press
引用: Human Reproduction, 21(10), pp.2545-2554; 2006
抄録: Background: The association between uterine myoma and infertility is still controversial. The anatomical defect of endometrium by uterine fibroids could be a factor for reducing pregnancy rates and increasing miscarriage rates. However, pregnancy and implantation rates were found to be significantly lower in women with intramural myomas (IMMs), when there was no deformity of uterine cavity. This could be due to other biological factors such as increased accumulation of inflammatory cells within fibroid tissue and corresponding endometrium that might impair fertility. Therefore, we tried to investigate the pattern of macrophage (Mψ) accumulation in different uterine fibroids and the production of chemokine and prostaglandin (PG) by these tissues. Methods: The selection criteria of uterine fibroids were based on the classification of European Society of Hysteroscopy. Biopsy specimens were collected from respective nodules and autologous endometrium of 20 women with submucosal myoma (SMM), 29 women with IMM and 18 women with subserosal myoma (SSM). CD68 immunoreactive Mφs were identified in these tissues by immunohistochemistry. A fraction of corresponding tissues were homogenized, and levels of monocyte chemotactic protein-1 (MCP-1) and PGF 2α were measured by enzyme-linked immunosorbent assay (ELISA). Results: Mφ infiltration in the myoma nodule and corresponding endometrium of women with SMM and IMM was significantly higher than that of women with SSM or control women (P < 0.01 and P < 0.05, respectively). This tissue accumulation of inflammatory cells was independent of the sizes of the myoma nodules and phases of menstrual cycle. The tissue concentration of MCP-1 corresponded to increased Mφ infiltration and was significantly higher in women with SMM and IMM than that in women with SSM (P < 0.05 for each). A positive correlation was observed between MCP-1 concentration and accumulated Mψ numbers in the endometrium of women with SMM and IMM but not in women with SSM. The tissue levels of PGF2α were also significantly higher in the nodule and corresponding endometrium of women with SMM and IMM than that in SSM or control women (P < 0.05 for each). Conclusions: Higher production of MCP-1 could be responsible for the increased accumulation of Mψ in women with SMM and IMM. The augmented inflammatory reaction in endometrium and increased PGF 2α levels might be detrimental to reproductive outcome in women with SMM or IMM.
キーワード: Infertility / Macrophages / Monocyte chemotactic protein-1 / Prostaglandin F2α / Uterine myoma
URI: http://hdl.handle.net/10069/34521
ISSN: 02681161
DOI: 10.1093/humrep/del205
権利: © 2006 Oxford University Press. / This is a pre-copy-editing, author-produced PDF of an article accepted for publication in Human Reproduction following peer review. The definitive publisher-authenticated version Human Reproduction, 21(10), pp.2545-2554; 2006 is available online at: http://dx.doi.org/10.1093/humrep/del205
資料タイプ: Journal Article
原稿種類: author
出現コレクション:030 学術雑誌論文

引用URI : http://hdl.handle.net/10069/34521



Valid XHTML 1.0! Copyright © 2006-2015 長崎大学附属図書館 - お問い合わせ Powerd by DSpace