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Regulation of hepatocyte growth factor by basal and stimulated macrophages in women with endometriosis

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Title: Regulation of hepatocyte growth factor by basal and stimulated macrophages in women with endometriosis
Authors: Khan, Khaleque Newaz / Masuzaki, Hideaki / Fujishita, Akira / Kitajima, Michio / Tomoko, Kohno / Sekine, Ichiro / Matsuyama, Toshifumi / Ishimaru, Tadayuki
Issue Date: Jan-2005
Publisher: Oxford University Press
Citation: Human Reproduction, 20(1), pp.49-60; 2005
Abstract: Background: The different macromolecules as secreted by macrophages (M ) in the pelvic environment are believed to enhance the growth of endometriosis. However, the possible mediator that stimulates M for the production of different growth factors is not well described. Therefore, we investigated the possible production of hepatocyte growth factor (HGF) by the basal and lipopolysaccharide (LPS)-stimulated M derived from women with or without endometriosis. Methods: Using primary culture and 4-well chamber slides, adherent M immunoreactive to CD68 were isolated from the peritoneal fluid (PF) of 20 infertile women with endometriosis and 12 women without endometriosis. The proliferation of basal and LPS-treated M was investigated by the dimethylthiazole tetrazolioum bromide (MTT) assay. The production of HGF in the culture media of basal and LPS-stimulated M was examined by enyme-linked immunosorbent assay. The expression of mRNA for HGF and its receptor, c-Met, in the M was investigated by RT-PCR. The effect of HGF on the growth of endometrial cells and M was analysed by bromodeoxyuridine (BrdU) incorporation. Results: A >100 % increase in the proliferation of peritoneal M derived from women with endometriosis, and particularly of those harbouring dominant red lesions, was observed after treatment with LPS (P < 0.05). A 4- and 3-fold increase in the production of HGF was observed by the LPS-treated M derived from women with stage I-II endometriosis and stage III-IV endometriosis, respectively, when compared with non-LPS-treated M (P < 0.001). At the transcriptional level, we found a 5-fold increase in HGF mRNA expression in LPS-treated M versus basal M in women with endometriosis (P < 0.001). The BrdU incorporation study indicates that 10-100 ng/ml of HGF enhanced the growth of endometrial epithelial cells, stroma and M (∼50% increase) derived from women with endometriosis (all P < 0.05). Conclusion: LPS could be an inflammatory mediator of macrophage stimulation in the pelvic microenvironment. Besides mesenchymal cells, HGF is also produced by peritoneal M and is possibly involved in the growth of endometriosis.
Keywords: Cell growth / Endometriosis / Hepatocyte growth factor / Lipopolysaccharide / Macrophage
URI: http://hdl.handle.net/10069/34522
DOI: 10.1093/humrep/deh525
Rights: © European Society of Human Reproduction and Embryology 2004; all rights reserved. / This is a pre-copy-editing, author-produced PDF of an article accepted for publication in Human Reproduction following peer review. The definitive publisher-authenticated version Human Reproduction, 20(1), pp.49-60; 2005 is available online at: http://dx.doi.org/10.1093/humrep/deh525
Type: Journal Article
Text Version: author
Appears in Collections:Articles in academic journal

Citable URI : http://hdl.handle.net/10069/34522

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