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Rat hepatocyte spheroids formed on temperature-responsive PIPAAm polymer-grafted surface maintain long-term differentiated hepatocyte function


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Title: Rat hepatocyte spheroids formed on temperature-responsive PIPAAm polymer-grafted surface maintain long-term differentiated hepatocyte function
Authors: Tomonaga, Tetsuo / Soyama, Akihiko / Yamanouchi, Kosho / Takatsuki, Mitsuhisa / Kuroki, Tamotsu / Koji, Takehiko / Eguchi, Susumu
Issue Date: Jul-2014
Publisher: Nagasaki University School of Medicine / 長崎大学医学部
Citation: Acta medica Nagasakiensia, 59(1), pp.1-6; 2014
Abstract: Background. Although the development of a practicable culture method to maintain long-term hepatocyte function is highly desirable, hepatocytes lose their liver-specific functions rapidly on regular collagen-coated dishes. In this study, we evaluated the efficacy of a special cell culture dish coated with the temperature-responsive polymer poly(N-isopropylacrylamide) (PIPAAm), and observed the morphological and functional changes of hepatocytes on changing the amount of polymer. Methods. Culture plates with varying amounts of PIPAAm polymer (1.5~3.5 times that in the commercially available temperatureresponsive polymer-containing dish (UpCell®, CellSeed, Tokyo, Japan)) were prepared. All dishes were enhanced by adding a layer of rat tail collagen I at a dose of 600 μg/dish for 3 hours. Hepatocytes isolated from Sprague-Dawley rats were cultured in these static culture systems. Morphologic changes and liver-specific functions were evaluated. Results. On day 7 of culture, spheroid formation of hepatocytes was observed in the high-polymer group, with the presence of glycogen and albumin in the spheroid. In the high-polymer group, the rate of albumin production was significantly higher than in the low-polymer group until day 14 of culture (P<0.001). Conclusion. The spheroid formation of hepatocytes cultured in the presence of a high level of PIPAAm showed the long-term maintenance of liver-specific functions in vitro.
Keywords: Hepatocyte / Temperature-responsive Surface / Spheroid / PIPAAm
URI: http://hdl.handle.net/10069/34694
ISSN: 00016055
Type: Departmental Bulletin Paper
Text Version: publisher
Appears in Collections:Volume 59, No. 1

Citable URI : http://hdl.handle.net/10069/34694

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