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Nutritional effects on the visual system of the rotifer Brachionus plicatilis sensu stricto (Rotifera: Monogononta)

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タイトル: Nutritional effects on the visual system of the rotifer Brachionus plicatilis sensu stricto (Rotifera: Monogononta)
著者: Kim, Hee-Jin / Sawada, Chihona / Rhee, Jae-Sung / Lee, Jae-Seong / Suga, Koushirou / Hagiwara, Atsushi
発行日: 2014年11月
出版者: Elsevier
引用: Journal of Experimental Marine Biology and Ecology, 460, pp.177-183; 2014
抄録: Rotifers have a light sensor called "eyespot" which is expected to be composed of rhodopsin. Based on the molecular feature of rhodopsin as regenerated with 11-cis-retinal, we hypothesized that phototactic behavior should be affected by the nutritional level of food; especially vitamin A availability. This study intended to address the following questions on the nutritional effects of using baker's yeast (Saccharomyces cerevisiae) and Nannochloropsis oculata: how does diet affect the pigmented area and absorbance of the eyespot, and how do these changes characterize phototactic behavior and population growth in the monogonont rotifer Brachionus plicatilis sensu stricto. The pigmented area of the eyespot decreased to 14.7μm2 with baker's yeast while it was maintained at the initial size of 82.9μm2 with N. oculata. Maximum absorbance of the eyespot was observed at a range of 470 to 525nm in the initial rotifers and it was not significantly changed with diet type and culture day. The value of the maximum absorbance was maintained with N. oculata, while it rapidly decreased on day 10 with baker's yeast. Stronger positive phototaxis with N. oculata was observed under lower light intensity (0.1 and 0.5Wm-2) at 470nm. On the other hand, phototaxis with baker's yeast became weak and no phototactic reactions were observed under the same lighting condition. From the genomic DNA database of rotifers, 12 putative opsin-relevant genes were identified. These results corroborate the hypothesis that rhodopsin is the visual pigment in the rotifer eyespot. Lack of vitamin A with baker's yeast should induce reduction of the pigmented area and the sensitivity of the rotifer eyespot resulting in weak phototaxis. The population growth of rotifers showed different patterns related to the food type and light intensity. The lowest population growth (0.33-0.37day-1) was shown with baker's yeast diet at 0.5Wm-2. This phenomenon may be significantly related to malnutrition on baker's yeast which is deficient not only in vitamin A but also in fatty acids, vitamin B12 and its derivatives.
キーワード: Eyespot / Nutrient / Phototaxis / Population growth / Rotifera
URI: http://hdl.handle.net/10069/34760
ISSN: 00220981
DOI: 10.1016/j.jembe.2014.07.005
権利: © 2014 Elsevier B.V. / NOTICE: this is the author’s version of a work that was accepted for publication in Journal of Experimental Marine Biology and Ecology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Journal of Experimental Marine Biology and Ecology, 460, (2014)
資料タイプ: Journal Article
原稿種類: author
出現コレクション:085 学術雑誌論文

引用URI : http://hdl.handle.net/10069/34760



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