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Production of macrophage activating factors by the mitogen-stimulated lymphocytes of Japanese parrotfish (Oplegnathus fasciatus) and the properties of this factor


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Title: Production of macrophage activating factors by the mitogen-stimulated lymphocytes of Japanese parrotfish (Oplegnathus fasciatus) and the properties of this factor
Other Titles: イシダイのマイトジェン刺激リンパ球によるマクロファージ活性因子の産生と本因子の特性
Authors: Xiao, Ning / Yagi, Motoaki / Liang, Jia / Miyazaki, Riho / Wang, Junjie / Taniyama, Shigeto / Tachibana, Katsuyasu
Authors (alternative): 肖, 寧 / 八木, 基明 / 梁, 佳 / 宮﨑, 里帆 / 王, 俊杰 / 谷山, 茂人 / 橘, 勝康
Issue Date: Mar-2013
Publisher: 長崎大学水産学部 / The Faculty of Fisheries, Nagasaki University
Citation: 長崎大学水産学部研究報告, 94, pp.9-15; 2013
Abstract: Spleen lymphocytes were prepared from Japanese parrotfish (Oplegnathus fasciatus) and incubated with 100 mg/ml of Concanavalin A bound to Sepharose 4B beads for 48h, and then the stimulated cell supernatants were collected. Thyoglycolate induced, allogenic, peritoneal macrophages were incubated with or without stimulated cell supernatants. Then, the phagocytic activity against inactivated yeast and peroxidase production were measured. Stimulated cell supernatants increased the phagocytic activity and the peroxidase production of allogenic peritoneal macrophages. The activities of these stimulated cell supernatants were lost completely when they were heated at 56℃ for 30 min or dialyzed against a pH 2 buffer for 24h-similar to IFN-γ. Stimulated cell supernatants did not increase phagocytic activity and peroxidase production of heterogenic mouse peritoneal macrophages. These findings suggest that the spleen lymphocytes of Japanese parrotfish can produce a macrophage activating factor in vitro with Concanavalin A bound to Sepharose 4B beads, and that this factor may be IFN-γ. / イシダイの脾臓細胞中のリンパ球をコンカナバリンA(Con A)セファロース4B とともに48時間培養し,その上清によるマクロファージ活性化を検討し,以下の結果を得た。Con A 培養上清で培養したイシダイ腹腔マクロファージの貪食能と細胞内のペルオキシダーゼ活性の両機能は共に亢進していた。イシダイCon A 培養上清をホ乳類でINF-γ 失活処理とされている熱(56℃)あるいは酸(pH2)処理を行ったところ,マクロファージの活性化が消失した。イシダイCon A 培養上清の腹腔マクロファージ活性化の種特異性について,マウス腹腔マクロファージを用いて検討したところ,後者は活性化能を持たず,イシダイCon A培養上清の種特異性が存在した。 以上のことより,養殖イシダイ脾臓細胞中のリンパ球は,Con A で培養するとINF-γ を産生すると推察され,イシダイの魚体内でINF-γ,マクロファージの免疫防御機構の存在が示唆された。
Keywords: peritoneal macrophage / phagocytic activity / IFN-γ / spleen lymphocytes / Japanese parrotfish (Oplegnathus fasciatus) / Concanavalin A
URI: http://hdl.handle.net/10069/34802
ISSN: 05471427
Type: Departmental Bulletin Paper
Text Version: publisher
Appears in Collections:No. 94

Citable URI : http://hdl.handle.net/10069/34802

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