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NAOSITE : Nagasaki University's Academic Output SITE > Faculty of Fisheries > Bulletin > Bulletin of the Faculty of Fisheries > No. 94 >

走査型電子顕微鏡を用いた魚類普通筋細胞外マトリックス観察試料作製における固定方法の検討


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Title: 走査型電子顕微鏡を用いた魚類普通筋細胞外マトリックス観察試料作製における固定方法の検討
Other Titles: Preparation of the extracellular matrix of fish ordinary muscle for scanning electron microscopy
Authors: 郭, 菲菲 / 梁, 佳 / 宮崎, 里帆 / 王, 俊杰 / 谷山, 茂人 / 橘, 勝康
Authors (alternative): Guo, Feifei / Liang, Jia / Miyazaki, Riho / Wang, Junjie / Taniyama, Shigeto / Tachibana, Katsuyasu
Issue Date: Mar-2013
Publisher: 長崎大学水産学部 / The Faculty of Fisheries, Nagasaki University
Citation: 長崎大学水産学部研究報告, 94, pp.25-28; 2013
Abstract: Aldehydes are the most common prefixation reagents to prepare specimens for scanning electron microscopy. In this study, scanning electron microscopic observations were made on the morphology of the extracellular matrix of carp ordinary muscle prepared using various prefixation reagents and fixation times. The structure of collagen fibers and its networks in extracellular matrix were more clear ly observed on day 5 than on day 2, when prefixation solution A (4% paraformaldehyde, 0.1 M sodium phosphate buffer, pH 7.2) was used, although honeycomb structure were not preserved during both periods. Prefixation of the extracellular matrix with solution B (2.5% glutaraldehyde, 0.1 M sodium phosphate buffer, pH 7.2) on days 2 and 5 preserved honeycomb structure with rough network, and some cellular debris remained. Prefixation of the extracellular matrix with solution C (2% paraformaldehyde, 2.5% glutaraldehyde, 0.1 M sodium phosphate buffer, pH 7.2) on day 5 preserved fine honeycomb structure with fine network without any cellular debris. These results suggested that prefixation for 5 days with solution C (2% paraformaldehyde, 2.5% glutaraldehyde, 0.1 M sodium phosphate buffer, pH 7.2) is the most suitable method of preparing extracellular matrix of fish ordinary muscle for scanning electron microscopy.
Keywords: パラホルムアルデヒド / paraformaldehyde / グルタルアルデヒド / glutaraldehyde / 普通筋 / ordinary muscle / 前固定 / prefixation / 走査型電子顕微鏡 / scanning electron microscopy
URI: http://hdl.handle.net/10069/34804
ISSN: 05471427
Type: Departmental Bulletin Paper
Text Version: publisher
Appears in Collections:No. 94

Citable URI : http://hdl.handle.net/10069/34804

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