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Reevaluation of bactericidal, cytotoxic, and macrophage-stimulating activities of commercially available Fucus vesiculosus fucoidan

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タイトル: Reevaluation of bactericidal, cytotoxic, and macrophage-stimulating activities of commercially available Fucus vesiculosus fucoidan
著者: Nishiguchi, Tomoki / Jiang, Zedong / Ueno, Mikinori / Takeshita, Satoshi / Cho, Kichul / Roh, Seong Woon / Kang, Kyong-Hwa / Yamaguchi, Kenichi / Kim, Daekyung / Oda, Tatsuya
発行日: 2014年 9月
出版者: The Korean Society of Phycology
引用: ALGAE, 29(3), pp.237-247; 2014
抄録: Polysaccharides prepared from marine algae sometimes contain contaminants such as polyphenols and endotoxins that may mislead their bona fide biological activities. In this study, we examined bioactive contaminants in commercially available fucoindan from Fucus vesiculosus, along with ascophyllan and fucoidan from Ascophyllum nodosum. F. vesiculosus fucoidan inhibited the growth of Vibrio alginolyticus in a concentration-dependent manner (0-1,000 μg mL−1). However, the antibacterial activity of the fucoidan significantly reduced after methanol-extraction, and the methanol-extract showed a potent antibacterial activity. The extract also showed cytotoxicity to RAW264.7 and U937 cells, and induced apoptotic nuclear morphological changes in U937 cells. These results suggest that the antibacterial activity of the fucoidan is partly due to the methanol-extractable contaminants that can also contribute to the cytotoxicity on RAW264.7 and U937 cells. On the other hand, the activities to induce secretion of nitric oxide and tumor necrosis factor-α from RAW264.7 cells were observed in the fucoidan even after methanol extraction, and the extract had no such activities. Our observations suggest that commercially available fucoidan should be purified prior to biochemical use.
キーワード: Antibacterial activity / Contaminants / Cytotoxic activity / Fucoidan / Fucus vesiculosus
URI: http://hdl.handle.net/10069/34882
ISSN: 12262617
DOI: 10.4490/algae.2014.29.3.237
権利: © 2014 The Korean Society of Phycology / This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
資料タイプ: Journal Article
原稿種類: publisher
出現コレクション:080 学術雑誌論文

引用URI : http://hdl.handle.net/10069/34882



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