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Early Staphylococcal Biofilm Formation on Solid Orthopaedic Implant Materials: In Vitro Study


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Title: Early Staphylococcal Biofilm Formation on Solid Orthopaedic Implant Materials: In Vitro Study
Authors: Koseki, Hironobu / Yonekura, Akihiko / Shida, Takayuki / Yoda, Itaru / Horiuchi, Hidehiko / Morinaga, Yoshitomo / Yanagihara, Katsunori / Sakoda, Hideyuki / Osaki, Makoto / Tomita, Masato
Issue Date: 9-Oct-2014
Publisher: Public Library of Science
Citation: PLoS ONE, 9(10), e107588; 2014
Abstract: Biofilms forming on the surface of biomaterials can cause intractable implant-related infections. Bacterial adherence and early biofilm formation are influenced by the type of biomaterial used and the physical characteristics of implant surface. In this in vitro research, we evaluated the ability of Staphylococcus epidermidis, the main pathogen in implant-related infections, to form biofilms on the surface of the solid orthopaedic biomaterials, oxidized zirconium-niobium alloy, cobalt-chromium-molybdenum alloy (Co-Cr-Mo), titanium alloy (Ti-6Al-4V), commercially pure titanium (cp-Ti) and stainless steel. A bacterial suspension of Staphylococcus epidermidis strain RP62A (ATCC35984) was added to the surface of specimens and incubated. The stained biofilms were imaged with a digital optical microscope and the biofilm coverage rate (BCR) was calculated. The total amount of biofilm was determined with the crystal violet assay and the number of viable cells in the biofilm was counted using the plate count method. The BCR of all the biomaterials rose in proportion to culture duration. After culturing for 2-4 hours, the BCR was similar for all materials. However, after culturing for 6 hours, the BCR for Co-Cr-Mo alloy was significantly lower than for Ti-6Al-4V, cp-Ti and stainless steel (P<0.05). The absorbance value determined in the crystal violet assay and the number of viable cells on Co-Cr-Mo were not significantly lower than for the other materials (P>0.05). These results suggest that surface properties, such as hydrophobicity or the low surface free energy of Co-Cr-Mo, may have some influence in inhibiting or delaying the two-dimensional expansion of biofilm on surfaces with a similar degree of smoothness.
URI: http://hdl.handle.net/10069/34912
DOI: 10.1371/journal.pone.0107588
Rights: © 2014 Koseki et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Type: Journal Article
Text Version: publisher
Appears in Collections:Articles in academic journal

Citable URI : http://hdl.handle.net/10069/34912

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