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Chromatographic determination of low-molecular mass unsaturated aliphatic aldehydes with peroxyoxalate chemiluminescence detection after fluorescence labeling with 4-(N,N-dimethylaminosulfonyl)-7-hydrazino-2,1,3-benzoxadiazole


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Title: Chromatographic determination of low-molecular mass unsaturated aliphatic aldehydes with peroxyoxalate chemiluminescence detection after fluorescence labeling with 4-(N,N-dimethylaminosulfonyl)-7-hydrazino-2,1,3-benzoxadiazole
Authors: Ali, Marwa Fathy Bakr / Kishikawa, Naoya / Ohyama, Kaname / Mohamed, Horria Abdel-Mageed / Abdel-Wadood, Hanaa Mohamed / Mahmoud, Ashraf Mohamed / Imazato, Takahiro / Ueki, Yukitaka / Wada, Mitsuhiro / Kuroda, Naotaka
Issue Date: 15-Mar-2014
Publisher: Elsevier
Citation: Journal of Chromatography B, 953-954; pp.147-152; 2014
Abstract: A highly sensitive, selective and reproducible chromatographic method is described for determination of low-molecular mass unsaturated aliphatic aldehydes in human serum. The method combines fluorescent labeling using 4-(N,N-Dimethylaminosulfonyl)-7-hydrazino-2,1,3-benzoxadiazole with peroxyoxalate chemiluminescence. The derivatives were separated on a reversed-phase column C8 isocratically using a mixture of acetonitrile and 90mM imidazole-HNO3 buffer (pH 6.4, 1:1, % v/v). The calibration ranges were: 20-420nM for methylglyoxal, 16-320nM for acrolein, 15-360nM for crotonaldehyde and 20-320nM for trans-2-hexenal. The detection limits were ranged from 4.4 to 6.5nM (88-130fmol/injection), the recovery results were within the range of 87.4-103.8% and the intra and inter-day precision results were lower than 5.5%. The proposed validated method has been successfully applied to healthy, diabetic and rheumatic arthritis patients' sera with simple pretreatment method. In conclusion, this new method is suitable for routine analysis of large numbers of clinical samples for assessment of the oxidative stress state in patients.
Keywords: Fluorescence labeling / Lipid peroxidation / Oxidative stress / Peroxyoxalate chemiluminescence (PO-CL) / Serum analysis
URI: http://hdl.handle.net/10069/34986
ISSN: 15700232
DOI: 10.1016/j.jchromb.2014.02.009
Rights: © 2014 Published by Elsevier B.V. / NOTICE: this is the author’s version of a work that was accepted for publication in Journal of Chromatography B. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Journal of Chromatography B, 953-954, (2014)
Type: Journal Article
Text Version: author
Appears in Collections:Articles in academic journal

Citable URI : http://hdl.handle.net/10069/34986

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