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Influence of pH on Extracellular Matrix Preservation During Lung Decellularization


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Title: Influence of pH on Extracellular Matrix Preservation During Lung Decellularization
Authors: Tsuchiya, Tomoshi / Balestrini, Jenna L. / Mendez, Julio / Calle, Elizabeth A. / Zhao, Liping / Niklason, Laura E.
Issue Date: 1-Dec-2014
Publisher: Mary Ann Liebert Inc.
Citation: Tissue Engineering Part C: Methods, 20(12), pp.1028-1036; 2014
Abstract: The creation of decellularized organs for use in regenerative medicine requires the preservation of the organ extracellular matrix (ECM) as a means to provide critical cues for differentiation and migration of cells that are seeded onto the organ scaffold. The purpose of this study was to assess the influence of varying pH levels on the preservation of key ECM components during the decellularization of rat lungs. Herein, we show that the pH of the 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS)-based decellularization solution influences ECM retention, cell removal, and also the potential for host response upon implantation of acellular lung tissue. The preservation of ECM components, including elastin, fibronectin, and laminin, were better retained in the lower pH conditions that were tested (pH ranges tested: 8, 10, 12); glycosaminoglycans were preserved to a higher extent in the lower pH groups as well. The DNA content following decellularization of the rat lung was inversely correlated with the pH of the decellularization solution. Despite detectible levels of cyotoskeletal proteins and significant residual DNA, tissues decellularized at pH 8 demonstrated the greatest tissue architecture maintenance and the least induction of host response of all acellular conditions. These results highlight the effect of pH on the results obtained by organ decellularization and suggest that altering the pH of the solutions used for decellularization may influence the ability of cells to properly differentiate and home to appropriate locations within the scaffold, based on the preservation of key ECM components and implantation results.
Keywords: Decellularization / Lung / Tissue engineering / ECM (extracellular matrix) / DNA
URI: http://hdl.handle.net/10069/34991
ISSN: 19373384
DOI: 10.1089/ten.tec.2013.0492
Rights: ©2014 Mary Ann Liebert, Inc. / The final published version is available from Mary Ann Liebert, Inc., publishers at http://dx.doi.org/10.1089/ten.tec.2013.0492
Type: Journal Article
Text Version: author
Appears in Collections:Articles in academic journal

Citable URI : http://hdl.handle.net/10069/34991

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