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Preparation and characterization of surfactin-modified silica stationary phase for reversed-phase and hydrophilic interaction liquid chromatography


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Title: Preparation and characterization of surfactin-modified silica stationary phase for reversed-phase and hydrophilic interaction liquid chromatography
Authors: Ohyama, Kaname / Inoue, Yu / Kishikawa, Naoya / Kuroda, Naotaka
Issue Date: 5-Dec-2014
Publisher: Elsevier
Citation: Journal of Chromatography A, 1371, pp.257-260; 2014
Abstract: Surfactants are good candidates as selectors in mixed-mode reversed-phase liquid chromatography (RPLC)/hydrophilic interaction liquid chromatography (HILIC) because they contain both a hydrophobic and a hydrophilic moiety. Surfactin, a cyclic heptapeptide, is an efficient biosurfactant produced by Bacillus subtilis that comprises seven amino acids and a β-hydroxyl fatty acid. A surfactin-modified silica (SMS) stationary phase was prepared by amide bond formation between amino groups on aminopropyl silica and the carboxylic acid groups of L-Glu and L-Asp residues in surfactin. The resulting SMS stationary phase was characterized in both RPLC and HILIC mode using different mobile phases. The SMS column was found to separate analytes in both modes. The retention of polar solutes exhibited "U-shaped" curves, depending on the acetonitrile content. "U-shaped" curves are an indicator of RPLC/HILIC mixed-mode retention behavior. The presence of hydrophobic and hydrophilic moieties in surfactin provides unique properties that allow the SMS column to be used for both RPLC and HILIC separations, simply by changing the mobile phase composition.
Keywords: Hydrophilic interaction / Mixed-mode / Reversed-phase / Surfactin
URI: http://hdl.handle.net/10069/35015
ISSN: 00219673
DOI: 10.1016/j.chroma.2014.10.073
Rights: © 2014 Elsevier B.V. / NOTICE: this is the author’s version of a work that was accepted for publication in Journal of Chromatography A. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Journal of Chromatography A, 1371, (2014)
Type: Journal Article
Text Version: author
Appears in Collections:Articles in academic journal

Citable URI : http://hdl.handle.net/10069/35015

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