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Proteomic profiling of antigens in circulating immune complexes associated with each of seven autoimmune diseases

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Title: Proteomic profiling of antigens in circulating immune complexes associated with each of seven autoimmune diseases
Authors: Ohyama, Kaname / Baba, Miyako / Tamai, Mami / Aibara, Nozomi / Ichinose, Kunihiro / Kishikawa, Naoya / Kawakami, Atsushi / Kuroda, Naotaka
Issue Date: Feb-2015
Publisher: Elsevier Inc.
Citation: Clinical Biochemistry, 48(3), pp.181-185; 2015
Abstract: Objective: Immune complexes (ICs) trigger humoral immune responses. Therefore, the identification of constituent antigens within ICs would have very different clinical significance than identification of free antigens. Design and methods: Here, we applied immune complexome analysis of serum to the study of seven major autoimmune diseases-anti-neutrophil cytoplasmic antibody-associated vasculitis, Takayasu's arteritis, mixed connective tissue disease, dermatomyositis, Sjögren's syndrome, systemic scleroderma, and systemic lupus erythematosus-and healthy donors to comprehensively identify antigens incorporated into circulating ICs and to find disease-specific antigens. Results: We identified 468 distinct IC-associated antigens using this method. Importantly, 62 of those antigens were disease-specific antigens, and there were at least three disease-specific antigens for each of the seven autoimmune diseases. Of the disease-specific antigens identified, coiled-coil domain-containing protein 158 and spectrin were identified as potential autoantigens important to SSc and SS pathogenesis, respectively; notable titin and spectrin autoantibodies are reportedly found in SSc and SS patients, respectively. Conclusion: Immune complexome analysis may be generally applicable to the study of the relationship between ICs and autoimmune diseases in animals and humans.
Keywords: Autoimmune disease / Immune complex / Immune complexome analysis / Tandem mass spectrometry
URI: http://hdl.handle.net/10069/35120
ISSN: 00099120
DOI: 10.1016/j.clinbiochem.2014.11.008
Rights: © 2014 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. / NOTICE: this is the author’s version of a work that was accepted for publication in Clinical Biochemistry. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Clinical Biochemistry, 48, 3, (2015)
Type: Journal Article
Text Version: author
Appears in Collections:Articles in academic journal

Citable URI : http://hdl.handle.net/10069/35120

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