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Quantitative RT-PCR evaluation of a rapid influenza antigen test for efficient diagnosis of influenza virus infection


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Title: Quantitative RT-PCR evaluation of a rapid influenza antigen test for efficient diagnosis of influenza virus infection
Authors: Tsushima, Yuko / Uno, Naoki / Sasaki, Daisuke / Morinaga, Yoshitomo / Hasegawa, Hiroo / Yanagihara, Katsunori
Issue Date: Feb-2015
Publisher: Elsevier
Citation: Journal of Virological Methods, 212, pp.76-79; 2015
Abstract: Influenza virus infection is diagnosed in most cases using a rapid influenza antigen diagnostic test (RIDT). However, false-negative results are a major concern. By contrast, the nucleic acid amplification test offers high sensitivity and therefore can aid the interpretation of negative RIDT results. In this study, influenza viral loads were quantified with quantitative reverse transcription-polymerase chain reaction (qRT-PCR) using viral suspensions left over after RIDT, and the performance of both methods was evaluated. qRT-PCR detected as few as 103copies/mL of influenza viruses A and B, whereas RIDT showed negative results for viral loads less than 107 and 105copies/mL of influenza viruses A and B, respectively. These results indicate that small quantities of the virus that cause false-negative RIDT results can be detected efficiently with qRT-PCR follow-up. In addition, influenza A virus subtype was determined using qRT-PCR.
Keywords: Influenza virus / Quantitative RT-PCR / Rapid influenza antigen detection test
URI: http://hdl.handle.net/10069/35305
ISSN: 01660934
DOI: 10.1016/j.jviromet.2014.10.019
Rights: © 2014 Elsevier B.V. / NOTICE: this is the author’s version of a work that was accepted for publication in Journal of Virological Methods. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Journal of Virological Methods, 212, (2015)
Type: Journal Article
Text Version: author
Appears in Collections:Articles in academic journal

Citable URI : http://hdl.handle.net/10069/35305

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