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Development of monoclonal antibodies against Plasmodium falciparum thioredoxin peroxidase 1 and its possible application for malaria diagnosis


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タイトル: Development of monoclonal antibodies against Plasmodium falciparum thioredoxin peroxidase 1 and its possible application for malaria diagnosis
著者: Hakimi, Hassan / Goto, Yasuyuki / Suganuma, Keisuke / Angeles, Jose Ma M. / Kawai, Satoru / Inoue, Noboru / Kawazu, Shin-ichiro
発行日: 2015年 7月
出版者: Academic Press Inc.
引用: Experimental Parasitology, 154, pp.62-66; 2015
抄録: Rapid diagnostic tests (RDTs) have been considered as an ideal alternative for light microscopy to detect malaria parasites especially in remote areas. The development and improvement of RDTs is an area of intensive research in the last decade. To date, few parasite proteins have been targeted in RDTs which are known to have certain deficiencies and made the researchers to look for other promising candidates to address this problem. Plasmodium falciparum thioredoxin peroxidase 1 (PfTPx-1) is abundantly expressed in the cytoplasm of the parasite and well conserved across Plasmodium species, making this antigen a promising target for malaria diagnosis. Several monoclonal antibodies (mAbs) were produced against PfTPx-1. The binding affinities of mAbs were measured. Several immunochromatographic tests (ICTs) were developed using different combination of mAbs. All mAbs showed promising affinities to be used for diagnosis. The sensitivities of ICTs were evaluated using recombinant PfTPx-1 whose results lead us to the preparation of 4 different ICTs. These tests showed positive reaction with P. falciparum in vitro culture supernatant indicating the release of PfTPx-1 during schizont rupture. Altogether, these findings suggest that PfTPx-1 is a promising biomarker to diagnose P. falciparum infection. However, the diagnostic performance of this antigen should be further validated using clinical samples.
キーワード: Malaria diagnosis / Plasmodium falciparum / Rapid diagnostic test / Thioredoxin peroxidase 1
URI: http://hdl.handle.net/10069/35455
ISSN: 00144894
DOI: 10.1016/j.exppara.2015.04.018
権利: © 2015 Elsevier Inc. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/
資料タイプ: Journal Article
原稿種類: author
出現コレクション:120 学術雑誌論文

引用URI : http://hdl.handle.net/10069/35455

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