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Determination of human serum semicarbazide-sensitive amine oxidase activity via flow injection analysis with fluorescence detection after online derivatization of the enzymatically produced benzaldehyde with 1,2-diaminoanthraquinone


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タイトル: Determination of human serum semicarbazide-sensitive amine oxidase activity via flow injection analysis with fluorescence detection after online derivatization of the enzymatically produced benzaldehyde with 1,2-diaminoanthraquinone
著者: El-Maghrabey, Mahmoud H. / Kishikawa, Naoya / Ohyama, Kaname / Imazato, Takahiro / Ueki, Yukitaka / Kuroda, Naotaka
発行日: 2015年 6月30日
出版者: Elsevier B.V.
引用: Analytica Chimica Acta, 881, pp.139-147; 2015
抄録: A fast, simple, and sensitive flow injection analysis method was developed for the measurement of semicarbazide-sensitive amine oxidase (SSAO) activity in human serum. Benzaldehyde, generated by the action of SSAO after incubation of serum with benzylamine, was derivatized with a novel aromatic aldehyde-specific reagent (1,2-diaminoanthraquinone) and the fluorescent product was measured by fluorescence detection at excitation and emission wavelengths of 390 and 570nm, respectively. Serum SSAO activity was defined as benzaldehyde (nmol) formed per milliliter serum per hour. The method was linear over SSAO activity of 0.2-150.0nmolmL-1h-1 with a detection limit of 0.06nmolmL-1h-1. The %RSD of intra-day and inter-day precision did not exceed 9.4% and the accuracy ranged from -6.5 to -0.6%. The method was applied for the determination of the serum SSAO activity in healthy controls (C, n=24) and diabetes mellitus patients (DM, n=18). It was demonstrated that the activity (mean±SE) of SSAO in diabetics sera was significantly higher than that in healthy subjects' ones (DM; 73.3±1.8nmolmL-1h-1 vs C; 58.9±2.2nmolmL-1h-1, P<0.01).
キーワード: 1,2-Diaminoanthraquinone / Benzaldehyde / Flow injection analysis / Fluorescence detection / Semicarbazide-sensitive amine oxidase activity
URI: http://hdl.handle.net/10069/35501
ISSN: 00032670
DOI: 10.1016/j.aca.2015.04.006
権利: © 2015 Elsevier B.V. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/
資料タイプ: Journal Article
原稿種類: author
出現コレクション:050 学術雑誌論文

引用URI : http://hdl.handle.net/10069/35501

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