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Endocytosis of Multiwalled Carbon Nanotubes in Bronchial Epithelial and Mesothelial Cells


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Title: Endocytosis of Multiwalled Carbon Nanotubes in Bronchial Epithelial and Mesothelial Cells
Authors: Maruyama, Kayo / Haniu, Hisao / Saito, Naoto / Matsuda, Yoshikazu / Tsukahara, Tamotsu / Kobayashi, Shinsuke / Tanaka, Manabu / Aoki, Kaoru / Takanashi, Seiji / Okamoto, Masanori / Kato, Hiroyuki
Issue Date: 2015
Publisher: Hindawi Publishing Corporation
Citation: BioMed Research International, 2015, 793186; 2015
Abstract: Bronchial epithelial cells and mesothelial cells are crucial targets for the safety assessment of inhalation of carbon nanotubes (CNTs), which resemble asbestos particles in shape. Intrinsic properties of multiwalled CNTs (MWCNTs) are known to cause potentially hazardous effects on intracellular and extracellular pathways. These interactions alter cellular signaling and affect major cell functions, resulting in cell death, lysosome injury, reactive oxygen species production, apoptosis, and cytokine release. Furthermore, CNTs are emerging as a novel class of autophagy inducers. Thus, in this study, we focused on the mechanisms of MWCNT uptake into the human bronchial epithelial cells (HBECs) and human mesothelial cells (HMCs). We verified that MWCNTs are actively internalized into HBECs and HMCs and were accumulated in the lysosomes of the cells after 24-hour treatment. Next, we determined which endocytosis pathways (clathrin-mediated, caveolae-mediated, and macropinocytosis) were associated with MWCNT internalization by using corresponding endocytosis inhibitors, in two nonphagocytic cell lines derived from bronchial epithelial cells and mesothelioma cells. Clathrin-mediated endocytosis inhibitors significantly suppressed MWCNT uptake, whereas caveolae-mediated endocytosis and macropinocytosis were also found to be involved in MWCNT uptake. Thus, MWCNTs were positively taken up by nonphagocytic cells, and their cytotoxicity was closely related to these three endocytosis pathways.
URI: http://hdl.handle.net/10069/35621
ISSN: 23146133
DOI: 10.1155/2015/793186
Rights: © 2015 Kayo Maruyama et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Type: Journal Article
Text Version: publisher
Appears in Collections:Articles in academic journal

Citable URI : http://hdl.handle.net/10069/35621

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