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Castalagin Exerts Inhibitory Effects on Osteoclastogenesis Through Blocking a Broad Range of Signaling Pathways with Low Cytotoxicity

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Title: Castalagin Exerts Inhibitory Effects on Osteoclastogenesis Through Blocking a Broad Range of Signaling Pathways with Low Cytotoxicity
Authors: Iwatake, Mayumi / Okamoto, Kuniaki / Tanaka, Takashi / Tsukuba, Takayuki
Issue Date: Jun-2015
Publisher: John Wiley and Sons Ltd
Citation: Phytotherapy Research, 29(6), pp.917-924; 2015
Abstract: Castalagin is a rare plant polyphenol that is classified as a hydrolyzable tannin. Although it has antioxidant, antitumorigenic, and leishmanicidal effects, the utility of castalagin against bone diseases remain to be elucidated. Here, we investigated the effects of castalagin on the differentiation of osteoclasts (OCLs), multinucleated bone-resorbing cells. After stimulation with receptor activator of nuclear factor kappa-B ligand (RANKL), the formation of OCLs from bone marrow-derived macrophages was significantly inhibited by castalagin even at 1-μM. However, castalagin displayed little cytotoxicity at a higher concentration of 50-μM. The effects of castalagin on intracellular signaling during OCL differentiation showed that castalagin suppresses RANKL-stimulated phosphorylation of major signaling pathways including protein kinase B (Akt), extracellular signal-regulated kinase, Jun N-terminal kinase, p38 mitogen-activated protein kinases, and inhibitor of nuclear factor kappa B alpha. Moreover, following castalagin treatment, the protein levels of nuclear factor of activated T-cells, cytoplasmic 1, a master regulator for OCL differentiation, and NF-κB were decreased. Thus, castalagin exerts inhibitory effects on osteoclastogenesis through blockage of a broad range of signaling pathways, but has low cytotoxicity.
Keywords: castalagin / NFATc1 / osteoclasts / RANKL
URI: http://hdl.handle.net/10069/35624
ISSN: 0951418X
DOI: 10.1002/ptr.5333
Rights: © 2015 John Wiley & Sons, Ltd. / This is the peer reviewed version of the following article: Phytotherapy Research, 29(6), pp.917-924; 2015, which has been published in final form at http://dx.doi.org/10.1002/ptr.5333 This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.
Type: Journal Article
Text Version: author
Appears in Collections:Articles in academic journal

Citable URI : http://hdl.handle.net/10069/35624

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