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Existence of Subserotypes in Streptococcus parauberis Serotype I


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Title: Existence of Subserotypes in Streptococcus parauberis Serotype I
Authors: Kanai, Kinya / Tu, Chuandeng / Katayama, Naoki / Suga, Koushirou
Issue Date: 30-Jun-2015
Publisher: 日本魚病学会 / Japanese Society of Fish Pathology
Citation: 魚病研究, 50(2), pp.75-80; 2015
Abstract: Streptococcus parauberis is the etiologic agent of streptococcosis in Japanese flounder Paralichthys olivaceus. Two serotypes, termed serotypes I and II, are known among the Japanese S. parauberis isolates. In the course of serodiagnosis, we found several strains that did not agglutinate with rabbit anti-serotype I or II sera. In this study, we investigated the serological and genetic relationships among the stocked S. parauberis strains including the non-agglutinating ones using a newly prepared rabbit antiserum against a non-agglutinating strain (NUF1071) as well as previously prepared antiserotype I and II sera, and pulsed-field gel electrophoresis (PFGE). An antiserum cross-absorption test and microtiter agglutination test revealed that the serotype I was divided into three subserotypes, tentatively designated Ia, Ib and Ic. The non-agglutinating strains belonged to the subserotype Ic. Of the 104 serotype I strains, 6, 91 and 7 strains belonged to subserotypes Ia, Ib and Ic, respectively. Formalin-killed cells of subserotype Ia and Ic strains were agglutinated with the anti-subserotype Ia serum (so far being used as an anti-serotype I serum) and Ic serum, respectively. Subserotype Ib strains were agglutinated with both sera. In PFGE analysis, the stocked 188 S. parauberis strains were divided into three clusters corresponding to subserotypes Ib/Ic, Ia and serotype II.
Keywords: Japanese flounder / Paralichthys olivaceus / PFGE / Serotype / Streptococcus parauberis / Subserotype
URI: http://hdl.handle.net/10069/35663
ISSN: 0388788X
DOI: 10.3147/jsfp.50.75
Rights: © 2015 The Japanese Society of Fish Pathology.
Type: Journal Article
Text Version: publisher
Appears in Collections:Articles in academic journal

Citable URI : http://hdl.handle.net/10069/35663

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