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Plasma Sex Steroid Levels and Steroidogenesis in the Gonad of the Self-fertilizing Fish Rivulus marmoratus

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タイトル: Plasma Sex Steroid Levels and Steroidogenesis in the Gonad of the Self-fertilizing Fish Rivulus marmoratus
著者: Minamimoto, Masako / Sakakura, Yoshitaka / Soyano, Kiyoshi / Akaba, Yoshiaki / Hagiwara, Atsushi
発行日: 2006年 2月
出版者: Springer Netherlands
引用: Environmental Biology of Fishes, 75(2), pp.159-166; 2006
抄録: The mangrove killifish, Rivulus marmoratus, is the only known self-fertilizing vertebrate. This species is sexually dimorphic; sexually mature individuals are either hermaphrodite or primary and secondary males. Although the mangrove killifish has a unique reproductive strategy, there has been no study on the reproductive endocrinology of this species. Thus we investigated plasma sex steroid hormone levels and steroidogenesis in the gonads of R. marmoratus by enzyme linked immunosorbent assay (ELISA). Plasma 17β-estradiol (E2) and 11-ketotestosterone (11-KT) were detected both in hermaphrodite and in primary male. Ovarian follicles (follicle-enclosed oocytes) from hermaphrodites, which were categorized into early yolk stage and late yolk stage, and testis tissue of primary males were cultured with different concentrations of 17α-hydroxyprogesterone (OHP) or testosterone (T) for 24 h. Production of T, E2, 11-KT and 17α-20 β-dihydroxy-4-pregnen-3-one (17α,20β-P) in the medium from tissue culture were measured by ELISA. Early and late ovarian follicles of hermaphrodites and testis pieces of primary males synchronously secreted E2, 11-KT, and 17α,20β-P following incubation with OHP or T. We conclude that both hermaphrodite and primary male of the mangrove killifish secrete estrogen, androgen, and progestin synchronously.
キーワード: Anterior cingulate cortexHermaphrodite / Mangrove killifish / Steroid hormone / Tissue culture
URI: http://hdl.handle.net/10069/35712
ISSN: 03781909
DOI: 10.1007/s10641-005-4504-6
権利: © Springer 2006. / The final publication is available at link.springer.com
資料タイプ: Journal Article
原稿種類: author
出現コレクション:080 学術雑誌論文

引用URI : http://hdl.handle.net/10069/35712



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