DSpace university logo mark
Advanced Search
Japanese | English 

NAOSITE : Nagasaki University's Academic Output SITE > School of Medicine > Articles in academic journal >

Rapid production of engineered human primary hepatocyte/fibroblast sheets


File Description SizeFormat
DB5_498.pdf1.63 MBAdobe PDFView/Open

Title: Rapid production of engineered human primary hepatocyte/fibroblast sheets
Authors: Sakai, Yusuke / Koike, Makiko / Soyama, Akihiko / Hidaka, Masaaki / Kuroki, Tamotsu / Eguchi, Susumu
Issue Date: Dec-2015
Publisher: Elsevier BV
Citation: Data in Brief, 5, pp.498-501; 2015
Abstract: This data article contains data related to the research article entitled "Vascularized subcutaneous human liver tissue from engineered hepatocyte/fibroblast sheets in mice," published in Biomaterials [1]. Engineered hepatocyte/fibroblast sheets (EHFSs) are used for construction of vascularized subcutaneous liver tissue without a pre-transplant vascularization procedures. Here, we described a rapid production technique of EHFSs by controlling fibroblast density and coating fetal bovine serum (FBS) onto temperature-responsive culture dishes (TRCDs). The human fibroblast monolayer formed on FBS-coated TRCDs within 1h when seeded at a high density (at least 1.56×105cells/cm2). The most rapid EHFS production was achieved soon after the adhesion of human primary hepatocytes onto the fibroblast layer.
Keywords: Cell sheet / Fibroblast / Human primary hepatocyte / Tissue engineering
URI: http://hdl.handle.net/10069/36036
ISSN: 23523409
DOI: 10.1016/j.dib.2015.09.044
Rights: © 2015 Elsevier Inc. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
Type: Journal Article
Text Version: publisher
Appears in Collections:Articles in academic journal

Citable URI : http://hdl.handle.net/10069/36036

All items in NAOSITE are protected by copyright, with all rights reserved.

 

Valid XHTML 1.0! Copyright © 2006-2015 Nagasaki University Library - Feedback Powerd by DSpace